FastQCFastQC Report
Thu 22 Jan 2015
GS5082_AGTCAA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGS5082_AGTCAA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4524447
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGG50200.014.0152971
AGCTTTT175900.013.7725681
CTCCCCG5259.241861E-512.434191
AGCTTCG41700.012.0018261
AGCTTCC57500.011.8575321
GATCGGA19900.011.6635621
CTCTCGG7601.8280934E-611.4525431
AGCTTTG86850.011.4415541
CTATGTG19350.011.2453911
AGCTTAT82950.011.0176371
CTATTAA107900.010.8227771
CTACGCG5450.001756383510.6470131
AGCTTGA87150.010.4866671
GAGCACA24750.010.2517199
AGCTTGT68400.010.0739971
AGCTTTC84400.09.9689611
CTATTAG36050.09.8588251
AGCTTGC45400.09.585831
CTCCGTG7903.8819812E-49.1813641
AGCTTCA95600.09.1804041
Produced by FastQC (version 0.11.2)