FastQCFastQC Report
Thu 22 Jan 2015
AH8217-6h_GTGAAA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8217-6h_GTGAAA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52864258
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG88700.025.596182145
CGTCGGC56950.010.436719
CGTCGTG1196050.010.012724145
CGTGTAG983450.09.898166145
AGCTTGG1449850.09.8367921
AGCGTCG1376250.09.766356145
GCTTGGC452450.09.7420722
GTCGTGT1125200.09.714893145
GAGCGTC1503200.08.970509145
TGTAGGG959600.08.949854145
TCGTGTA1087100.08.927731145
GTGTAGG984200.08.903034145
AGAGCGT1636150.08.800186145
AGCTTGC1361900.08.7471091
AAGAGCG1850350.08.608608145
AGATCGG2683250.08.32885145
GATCGGA2511600.08.285802145
GTAGGGA899300.08.235228145
TCGGGTG71300.08.2347525
GCGTCGT1265300.08.168787140-144
Produced by FastQC (version 0.11.2)