FastQCFastQC Report
Thu 22 Jan 2015
AH8217-6h_GTGAAA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8217-6h_GTGAAA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52864258
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGC63800.011.9299939
GCGTCGG63750.011.4845168
ACACGTC1151450.09.847576145
AGCTTGG1481100.09.6467591
GCACACG1291700.09.519231145
AGCACAC1473950.09.394816145
CACACGT1274550.09.334464145
ACGTCTG1086400.09.0157795145
AGCTTGC1363400.09.0113461
GCTTGGC463150.08.7348182
CCCCACT238600.08.7239071
CACGTCT1134650.08.715456145
AGAGCAC1770600.08.611057145
TGCTCGC127500.08.586268145
CGTCTGA1017600.08.535274145
AGCGTCG90750.08.4670247
AGATCGG2698100.08.321869145
GATCGGA2534600.08.30377145
GCTTTGG914000.08.2495172
GAGCACA1640000.08.182769145
Produced by FastQC (version 0.11.2)