FastQCFastQC Report
Thu 22 Jan 2015
AH8057-6h_GTCCGC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8057-6h_GTCCGC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13810421
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATA274050.011.0326141
CTAAACA150200.010.2821011
CTATTCA159200.09.9285451
CTATAAT204150.09.9089351
CTATTAT279550.09.9077741
CTATATG126650.09.7323091
CTATTCT163350.09.6763051
CTATTCG63150.09.5296491
CTATTTA266150.09.3169081
CTAAGTG85800.09.2956041
CTATGAG59500.09.2612321
CTATTTG191250.09.2504011
CTAAAGG140200.09.2054311
CTATGCG28550.09.1425751
CTATTAG127950.09.1234231
CTAAGAG63800.09.0916251
CTATATT238000.09.047981
CTATTAA204150.08.9855211
CTAATAG141950.08.9387091
CTAAAGA215550.08.9139461
Produced by FastQC (version 0.11.2)