FastQCFastQC Report
Thu 22 Jan 2015
AH8057-6h_GTCCGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8057-6h_GTCCGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13810421
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA48850.021.8256321
GAGCACA79700.013.6425939
AGAGCAC87050.012.407428
CTATACG42850.011.0021261
ACGTCCG21350.010.59381830-34
CTAAACA144600.010.1822051
CTAATAG158800.010.0938271
CGTCCGC22500.09.98791830-34
CTAAAGA208600.09.9093381
CTATATG126200.09.8851491
CTATATA269250.09.643651
CTATTAT288400.09.4308351
ATCGGAA121600.09.3010852
CTCTTAG76700.09.267121
CTAAGAT101050.09.2590721
CTATACA107500.09.2432811
CTATATT239150.09.068071
CTATTAG121800.09.0512881
CTAAGGT66800.09.0118871
CTATTCG63800.08.867231
Produced by FastQC (version 0.11.2)