FastQCFastQC Report
Thu 22 Jan 2015
AH8049-6h_ACAGTG_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8049-6h_ACAGTG_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40595698
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATA753650.07.00387861
CTATACG127700.06.983791
CTATTTG555850.06.9134561
CTATAAT574150.06.88252931
CTATATG364550.06.8817041
CTAAACA424750.06.8622991
CTATACA323750.06.80833241
CTAAGTG245800.06.6370881
CTAAAGG424650.06.57364941
CTATTGG345800.06.56290771
CTATTCA476800.06.49334671
CTATATT681000.06.48406171
CTATTAG366400.06.29286771
CTATTAT806400.06.26699261
CTATTCT487850.06.2570951
CTATTTA729050.06.2257831
CTAAATG429800.06.2249651
CTATTTC755050.06.18425131
CCCCACT226850.06.1687261
CTAGTCT178100.06.1473791
Produced by FastQC (version 0.11.2)