FastQCFastQC Report
Thu 22 Jan 2015
AH8049-6h_ACAGTG_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH8049-6h_ACAGTG_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40595698
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA134650.07.6487541
CCCCACT204500.07.4833811
CTATACA306600.07.35693551
CTCCCCG41600.06.9738941
CTAAACA409350.06.7859821
CTATACG120000.06.7693271
CTCTTCG331450.06.69594861
CTAATAG462100.06.6234641
CTAAGTG259200.06.519731
CTATTAT848650.06.50376371
CTATTCA449850.06.3362681
CTATATA727000.06.2751621
AGCTTGG871850.06.1809571
CTATTTC776700.06.10707331
CTATATG360250.06.0801131
CTAAATG425400.06.0696161
CTATTTG567900.06.06639151
CTAAAGG429900.06.0398241
CTAAAGA581800.05.9713221
CTATAAT578850.05.9516351
Produced by FastQC (version 0.11.2)