FastQCFastQC Report
Thu 22 Jan 2015
AH6851-6h_GCCAAT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6851-6h_GCCAAT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63026367
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG186300.026.438654145
CGCCATC390500.021.102783145
GCGTCGG137750.019.69529145
CCGACCG62300.016.301308145
CGGTGGG165400.015.701096145
TCGCCGG95700.014.781043145
TGCCGGA367350.014.6637929
CGTCGGC112450.014.5064329
GATCGGG142100.013.9919861
CTGCCGG402150.013.3405998
GTTCGCC315650.013.191328145
TCGGGAG120950.012.7760183
ATCATGC534850.012.64056145
AGCTTAA1702000.012.6241381
GCTTGGC621600.012.41937352
CTCTCTG1012700.012.3746911
GCTGCCG445150.012.1170877
ACGCTCG611650.012.0072629
CCATCCT389700.011.63408145
CGGGAGA163900.011.3755614
Produced by FastQC (version 0.11.2)