FastQCFastQC Report
Thu 22 Jan 2015
AH6851-6h_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6851-6h_GCCAAT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63026367
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTGCTGCCGGAAATGCTCTCTGTTCAAAAAGCTTTTACACTCTTGA819260.1299868672424035No Hit
CTCTTCATAATGTAAGCTTATCTTTATCGAATCGTGTGAAAAACTACTAC734690.11656867355213416No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCATC421450.021.332518145
TGCCGGA673950.020.6956359
TCCCCTC218750.020.4805589
CTGCCGG718150.019.4520268
TCGACCG105600.019.0159159
CTCGGAC80900.018.6385927
GCGTCGG97300.018.4027378
GCTGCCG778650.018.2851077
TCTCTGC806250.017.7666912
CGTCGGC106900.017.2927429
AGCTTAA1985000.016.6989441
CCGACCG58200.016.3198145
AGCTTGC1673400.015.7513761
TGCTGCC946600.015.3165786
GTTCGCC347800.014.759451145
CTCTGCT1001100.014.5694723
GCTTCGT577950.014.017552
TCGGACC111400.013.5355658
TACCTCG163750.013.3762115
GCTTGGC701050.013.1183582
Produced by FastQC (version 0.11.2)