FastQCFastQC Report
Thu 22 Jan 2015
AH6851-6h_ATGTCA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6851-6h_ATGTCA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50820500
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG86000.025.557365145
CGTCGGC63300.012.0233969
AGCTTGG1362250.09.5805461
AGCTTGC1271950.09.40564251
GCTTGGC436100.09.1598132
CGTGTAG1067700.09.042753145
AGCGTCG1436200.09.020661145
CGTCGTG1260850.08.905933145
TCGGGTG62650.08.7932955
GCTTGTG472800.08.6328072
GAGCGTC1562950.08.456198145
TCTCGGG106750.08.426083145
GTGTAGG1067500.08.419287145
TCGTGTA1165950.08.398964145
GTCGTGT1196100.08.308544145
AGAGCGT1689650.08.002414145
GCTTACG337550.07.98961642
TGTAGGG1028950.07.9380894145
GCTTGCA536550.07.91787342
GTAGGGA975750.07.828196145
Produced by FastQC (version 0.11.2)