FastQCFastQC Report
Thu 22 Jan 2015
AH6851-6h_ATGTCA_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6851-6h_ATGTCA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50820500
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGC58800.012.6977389
GCGTCGG61550.010.8349348
AGCTTGG1414700.09.7922611
AGCTTGC1263650.09.6885691
GCTTGGC449750.09.2207842
ACACGTC1222000.08.786687145
GCACACG1350750.08.711338145
AGCACAC1531800.08.614114145
CACGTCT1206450.08.461251145
ACGTCTG1156950.08.447274145
GCTTGCA526700.08.4380122
CGTCTGA1084500.08.416615145
CACACGT1336750.08.379528145
AGAGCAC1812000.08.070287145
GAGCACA1688150.07.8979063145
GCTTTGG901600.07.8885662
CCCCACT242400.07.86931751
GATCGGA2502450.07.6282816145
TGCTCGC129450.07.560891145
GCTTACG339250.07.5225622
Produced by FastQC (version 0.11.2)