FastQCFastQC Report
Thu 22 Jan 2015
AH6851-4h_CTTGTA_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6851-4h_CTTGTA_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649862
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATCATCAGTAAGCTTTGACAAAAAGCATTGAGTAGCTAACTCTTCTA110920.12823325967512544No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG37300.072.953791
TCGGGAG30600.066.147763
CGGGAGA40900.065.453894
GAGGGGC35500.063.310639
ATCGGGA51000.053.4918672
GGGAGAG60900.051.2225155
GAGAGCG34150.044.5822377
GAGAGGG49750.042.1151167
GGAGAGC38400.040.592946
GAGCGGC48400.038.1977779
GGAGAGG57200.038.1516886
AGAGCGG53050.037.035418
AGAGGGG77800.034.0131238
GAACGGG32400.032.6989061
AAGGGGC38850.025.006749
TCGGGGG56300.023.40963635-39
ACGGGAG32800.022.3398323
AAGCGGC38150.021.8547889
AACGGGA50400.021.305972
GCGTCGG19350.020.620373145
Produced by FastQC (version 0.11.2)