FastQCFastQC Report
Thu 22 Jan 2015
AH6851-4h_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH6851-4h_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649862
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC912681.0551382207022493TruSeq Adapter, Index 12 (100% over 50bp)
ATCATCATCAGTAAGCTTTGACAAAAAGCATTGAGTAGCTAACTCTTCTA124340.14374795805990895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGC77250.023.558386145
ACGCTCG63650.022.7787089
CGTCGGC12800.022.6541359
CACGCTC68450.021.075348
GCGTCGG13300.020.7122338
TCCCCTC31850.020.0295289
CTCGGAC10550.019.23987
TCACGCT78750.019.2393657
CAGTAAG122050.017.8782989
TTCACGC86200.016.8196776
TCGACCG15100.016.8030689
CATCAGT137350.016.4144636
AGCTTGC227450.016.3914811
GAGCACA962550.016.1397329
AGAGCAC998950.015.573318
AGCTTAA292150.015.3682991
ATCAGTA154050.014.96443757
AGCGTCG18650.014.7706547
GAAGAGC1093400.014.2612096
TCATCAG162900.014.0701755
Produced by FastQC (version 0.11.2)