FastQCFastQC Report
Thu 22 Jan 2015
AH2640-6h_GTCCGC_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH2640-6h_GTCCGC_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33762160
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG76750.030.5402981
TCGGGAG70150.027.1998043
CGGGAGA92800.026.658544
GAGGGGC86900.021.1077029
GGGAGAG156300.018.14755
ATCGGGA136800.017.7683582
CTCGGGG42850.014.54953435-39
GAGAGCG79000.013.9494177
TCGGGGG86750.012.96951335-39
AGAGGGG181650.012.8516838
GAGAGGG147700.012.7624027
GATCGGA143100.012.6272521
TGCTCGC94500.011.28486145
AGCTTGG631550.010.9734911
GGGGGGC121100.010.8720140-44
GGGGGCG101150.010.78003340-44
TCACGGC103200.010.3269026
GGAGAGG194300.010.2983766
GGAGAGC107950.010.073986
CGGGGGG104900.09.95149835-39
Produced by FastQC (version 0.11.2)