FastQCFastQC Report
Thu 22 Jan 2015
AH2640-6h_GTCCGC_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAH2640-6h_GTCCGC_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33762160
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT877060.2597760332869698TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA229300.065.257921
GAGCACA288100.051.482919
AGAGCAC317400.046.7756588
ATCGGAA407700.037.3408932
TCGGAAG434600.034.9461983
CGGAAGA501100.030.1924514
GGAAGAG557500.027.294125
GAAGAGC603850.025.1027776
AAGAGCA743550.020.3864087
CGTCCGC148400.019.9416330-34
ACGTCCG153300.019.51230630-34
GTCCGCA174100.017.0895330-34
CCGCACA180200.016.54233635-39
CACGTCC183900.016.38382730-34
GCACACG188150.015.95132610-14
GCCGTCT203150.014.9946450-54
ACACGTC204100.014.9462910-14
AGTCACG209750.014.76614525-29
CATCTCG199850.014.2918940-44
GTCACGT219850.013.96906525-29
Produced by FastQC (version 0.11.2)