FastQCFastQC Report
Wed 12 Jan 2022
HF5W3AFX3_n02_as2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF5W3AFX3_n02_as2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11148997
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG661820.5936139367514405Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT422530.37898476427969263Illumina Single End PCR Primer 1 (100% over 31bp)
TCGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA314760.2823213603878447Illumina Single End PCR Primer 1 (100% over 34bp)
CGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT260820.23394032665001166Illumina Single End PCR Primer 1 (100% over 33bp)
CGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG175380.15730563027328825Illumina Single End PCR Primer 1 (100% over 32bp)
GGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT143860.12903402880097645Illumina Single End PCR Primer 1 (100% over 39bp)
TCGTGTAGGGAAAGAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT130880.1173917259104115Illumina Single End PCR Primer 1 (100% over 33bp)
CGTGTAGGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG123090.11040455029273036Illumina Single End PCR Primer 1 (100% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115790.10385687609387642No Hit
GGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC111710.10019735407588683Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)