FastQCFastQC Report
Wed 12 Jan 2022
HF5W3AFX3_n01_as2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF5W3AFX3_n01_as2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11148997
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC777130.6970402808432005Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT507170.45490190731955527Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC357630.32077324982686783Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA303930.27260748209009295Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC212730.19080640168797247Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG194110.17410534777253955Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG191320.17160288051023784TruSeq Adapter, Index 8 (100% over 38bp)
ACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA156890.14072117877509518Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC154570.13864027409819915Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA144880.12994890930547384Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT139590.12520408786548243TruSeq Adapter, Index 8 (100% over 37bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA136820.12271955943660223Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT134410.12055793000930937TruSeq Adapter, Index 8 (100% over 37bp)
CACTCTTTCCCTACACGACAGATCGGAAGAGCACACGTCTGAACTCCAGT124720.11186656521658407Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC115130.10326489459096634Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)