FastQCFastQC Report
Wed 12 Jan 2022
HF5W3AFX3_n01_as2-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF5W3AFX3_n01_as2-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11839031
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC231920.19589441061519308Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT178070.1504092691369758Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC121170.10234790330391061Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)