FastQCFastQC Report
Thu 28 Jul 2022
HF5FTDRX2_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF5FTDRX2_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210580219
Sequences flagged as poor quality0
Sequence length90
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA46973640.21249461836426575Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35157990.15904417174195298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAACCTCAACTCATATGGG33323980.15074766214579974Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTAGTTTTTATTTATTAAT23077780.10439693525548552Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)