FastQCFastQC Report
Thu 28 Jul 2022
HF5FTDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF5FTDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2210580219
Sequences flagged as poor quality0
Sequence length10
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCACGCATA55903683325.28914482247975No Hit
CACTTACCTG53240895524.084579714589402No Hit
CTAGACGATT52320347623.66815153338708No Hit
CTAGAGGTCA47764798221.607358009205093No Hit
AGATCTCGGT753378703.408058633315763Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG53729970.24305822307731417No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)