FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n02_HRBB6LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n02_HRBB6LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC76

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1417.72151898734177No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGATCT33.79746835443038Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG33.79746835443038No Hit
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGT22.5316455696202533Illumina Single End PCR Primer 1 (96% over 33bp)
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCGTCGTGTAGGG22.5316455696202533Illumina Single End PCR Primer 1 (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGTGGGGG11.2658227848101267No Hit
CTTTCTCCTCCACCTTGCCATAGAAGTAGATCGGAAGAGCGTCGTGTAGG11.2658227848101267Illumina Single End PCR Primer 1 (100% over 23bp)
AGATCGGAAGAGCGTCGTGTATGGAAAGAGTGTGCTCGAAGTGTATATCT11.2658227848101267Illumina Single End PCR Primer 1 (96% over 33bp)
TCTACTAAAACCATTCTGCCAGCATTAACCGTGAATGGAGATCGGAAGAG11.2658227848101267No Hit
GGGGGGGGGGGGGTAAGTAAATTAAAAAAATTAAAAAAAAAAAAATAAAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGGGGGGGTGGGGGTG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGGGGGGGGGGGGTGGGG11.2658227848101267No Hit
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGTGTAGA11.2658227848101267Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGTGGGGGTGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGTGGTTGG11.2658227848101267No Hit
GTTTTAACTGTTTATACGGCAATTCAAGATCGGAAGAGCGTCGTGTAGGG11.2658227848101267Illumina Single End PCR Primer 1 (100% over 24bp)
GGGGGGGTCGAACTAGCATAAAGAATAATTATTATTAATATAAAAAAAAA11.2658227848101267No Hit
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGT11.2658227848101267Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGCTGCTATTATATATAATATAAAAAGAAAAATTAAAAAT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGTGGGGGGGGGTGGTGTGGGT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGTAGGGAAATATATAGAGGTAAATTATTATTA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGTGGGGGGGGTGGGGGGGGG11.2658227848101267No Hit
AATTATGAAACTGCACGTGACGAGATTTACAAAGTCTTCGCTGACGAGAT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG11.2658227848101267No Hit
GGGGGGGGGGTCCGCGGGTATTTAAAAAAATTTTAAAAAAATTAAAAAAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTTGTGGGGGGGTGATGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGTGTGGTG11.2658227848101267No Hit
ACTTCCTGACCACCTGGCTCACCACGCCGATCGATGGCGAGACACTGTCG11.2658227848101267No Hit
TCGTAACTACGAAGATCGGAAGAGCGTCAGATCGGAAGAGCGTCGTGGAG11.2658227848101267Illumina Single End PCR Primer 1 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGTGTGGAGTTGTGGGGGGTGTGTTGTGTT11.2658227848101267No Hit
GAACTCAACTTAGGACCCTTACTAGTACCCAGGTGTACCTGGAGAGATCG11.2658227848101267No Hit
GGGGGGGGGGCGCCCCCATAAAATAAAAAAAAAAAAGAAAAAAAAAAAAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGTTGGGTTGTGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTTGTTGGGGGGGGGGGGGGTGTGGG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGTTGGGGGGGGGGGGGT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGTTGGGGAGGAGTTTTTGGGTGTGTTTTTTTG11.2658227848101267No Hit
GGGGGGGGTCCTACGCAGACTAATAAATAAAAACATCAAAATGAAAAGAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGTGGGGGGGGGGGTTGTGTG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGTGGGGGGGGTTGGGGGGTGATGGGGAGGGA11.2658227848101267No Hit
GTGACTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTCGAAGT11.2658227848101267Illumina Single End PCR Primer 1 (96% over 33bp)
AGACCAGAAATAAGAAACAAAGGCACCACAAAAAAAAGTTGAACCCCAAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGTGGGGGGTGGTGGTGGGG11.2658227848101267No Hit
GGGGGGGGGGCGCGTCCTGATAAATTCATACCGATATAAAAATAAAATTA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGTG11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGTGGGTGG11.2658227848101267No Hit
GGGGGGGGGGGGGGCGGGCGGGATGGACGACGTTGGGGCTATCCGTGTTT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGTGGTGGG11.2658227848101267No Hit
GCCGGATCAGGCCGCCCTGGAGAGCCGTCGCGCCAAAGACCGTGCACCCA11.2658227848101267No Hit
GGGGGGGGGGGCGACAGGAGAAAAGAAAAATAATAAAAAAAAAAAAAAAA11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGTTTTGTG11.2658227848101267No Hit
GGGGGGGTTATAAGAAAATATTATAAAAAATATAAAAAAAAATAAAAAAA11.2658227848101267No Hit
AGAGATGAGAGATATGAAGATACAACTTGAACTGCAGAATAGGGCTACCT11.2658227848101267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTTGGGTGTGTGGTGGTGGTTTGGTT11.2658227848101267No Hit
GGGGGGGGGGGGGCAGCGAGTGCAAAAAAATTAAGAAAGAATAAATAAAA11.2658227848101267No Hit
GGGGGGGGGGGGGGCGAGCTGACCTTAAAATATAATAAAAAAATAAAAAA11.2658227848101267No Hit
GGGGGGGGGGGGGTGGGGTTGACATTTACAACATATTCATTGTTAATCAT11.2658227848101267No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)