FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n02_HRBB5LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n02_HRBB5LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3785
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1062.8005284015852046No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGACCTGGTGTAGATCT381.0039630118890357Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGG100.26420079260237783No Hit
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGACCTGGTGT80.21136063408190223Illumina Single End PCR Primer 1 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG60.15852047556142668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGG50.13210039630118892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG50.13210039630118892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG40.10568031704095111No Hit
GCTTCACAGGCCTTCCAAGACGGCGCTAAGCGAGCGCGTCCAGTAATCAT40.10568031704095111No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGG40.10568031704095111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)