FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n02_HRBB5EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n02_HRBB5EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1393.7854030501089326No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCGGTGTAGATCT962.6143790849673203Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG80.2178649237472767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG70.1906318082788671No Hit
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCGTCGTGTAGGG70.1906318082788671Illumina Single End PCR Primer 1 (100% over 24bp)
GTTCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCGGTGT60.16339869281045752Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGG60.16339869281045752No Hit
CCAGTCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCTCGGT50.13616557734204793Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTG50.13616557734204793No Hit
GTTCTACAGAACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATCT40.10893246187363835Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGG40.10893246187363835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG40.10893246187363835No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGG40.10893246187363835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)