FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n02_HRB0073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n02_HRB0073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGGCGCCCAGCTTTGCGATCCGCTCCAGCAGCGCCGCCTGCGCTGG22.0No Hit
TACCAAAATAAAACCTAATTAGCAAGCCATATGATTCAGTCCATAAGTTT22.0No Hit
TTCCAACATCATCTACTTTGGCGCACGAAGCGGGAATGGACTGTGGAAGA11.0No Hit
ATAACTTTCCAATACCCGTCTCAGCGATGGAGTTCGACGGAGATCGGAAG11.0No Hit
GCGCGGCCCGTCGCCGAGGAGGTTGGCGACCGAGGCGGCGATGTGGTCGT11.0No Hit
ACTTGTCACGAGAGGCGGCCCATTGATTATCAGGAGACAGATAGGATGTA11.0No Hit
GTGTGAATGACGCGCAGACTAAGATCGGAAGAGCGTCGTGTAGGGAAAGA11.0Illumina Single End PCR Primer 1 (100% over 29bp)
ATGACCTGAATGCACTCTACACTTGCGAAGCCTTTCAAGAGGTGGTCGAC11.0No Hit
ATCCTATCCATTCTATATCATAAATCATAGCAAATAATCAAGATCGGAAG11.0No Hit
GACCAGATACCAACTCTGAGCCTTGTATCATTAGGTTTCCAACACAAGAG11.0No Hit
TCATTACCATCCAGTTCAAAGACGTTCCGCATTTCATTTTTCCGCCCGAA11.0No Hit
TACAATGACATTGTACAACAATTTGGAAAAGGGATGTAGCGCAGCTTGGT11.0No Hit
CCGTGGAGCCACCTCACTAGCTCCGCTTCGGCTCATTTGCTCGAGAGACT11.0No Hit
AATGGGCTATGCAAGAACTTGACAAACTAGAAAGCTTGATGAATGTCGAT11.0No Hit
GCACACGTAAAGGCATCAATCTTCCCTCCAGTCTGGAGATCGGAAGAGCG11.0No Hit
GTTCGGCGGAGGAGCTGTAGATGAAGATCGGAAGAGCGTCGTGTAGGGAA11.0Illumina Single End PCR Primer 1 (100% over 26bp)
ATTAATAGAATATAGTCTTTTGTATATTTGGTTAGAAAGGTTGTTGATGA11.0No Hit
CGTCCGATATTCTATCCAGGAGGACCTCAAGAAGGCGGGTCTTTATGTCG11.0No Hit
GTAGCCATGTTGGTCCATGCTGGCTCACATTGCAACATGACGCTAGAAAT11.0No Hit
ACAACACGCCATGTATACACCCTAATTAAAAAAACAATTCCTACCCAATA11.0No Hit
AGATCTTTTTCATGTTCTTCTCGAAGCAGCGATCGACCTCGGGGCTTTCC11.0No Hit
TGCTCACCTCACTCTGCTAGATTGTAGTTTTTGACCCCACAAGTGCCGTG11.0No Hit
CCGCGCGAGTCGCGAAGTGAGTTTTTGCCACGAACGCACCCAATACAGTA11.0No Hit
TACACCAAGAGCGGCCAGCACAGTGACGATCACCGCCGGCGCGCGCCACA11.0No Hit
ATGAGGACCAGGATACCCAAATGGGCTGTCGTTGTTCAACCCATTCGTCA11.0No Hit
TGTCTTGGGCAATTGACATACTGCTGTTTGAAGATCGGAAGAGCGTCGTG11.0No Hit
AGGCACCCGTCGAGGATTGGTTTGCAGACGCAAAATTGCCGCCCATGACA11.0No Hit
AGCTCGTGTGGGTCCTCCTCATCGTGCTGCTGCCCGTCGTCGGTGGCATC11.0No Hit
GTGAGCACAGGCATATTGAACGTATACTGATTAAGAACGTCCGGAATGTT11.0No Hit
CGATCCGGTCGTGTATGTCCCGGATAACGCGGCGGCCCACGTCGTCCACG11.0No Hit
CTTAACCCTGAAGTTCTTTGGAGATTGGCTTCCGAAAAAGAAGTTGCAAC11.0No Hit
CTCCTCTGGCTCCAAACAACGACTTATTCAAGAGTCCTGCACAAACTGCA11.0No Hit
TACAAGGGAAAAATAAATTCCATCCATCTTGCTGTAAAAATTTTTCCTAT11.0No Hit
ACGCCGTCAATTGCGTGGCTGCGGAGAGAATTGGCTGTCGGCACGGTTGG11.0No Hit
CCCGAGGATCCCTATGCCAGCCTGGCGCCGGCCGACCGCCTGGCCCGCGC11.0No Hit
ATGGTGCGGGAACGGCTGACGGCGGAATAGAAAACCACCTTACCAATCCA11.0No Hit
AGCCGCGCCGAGGCCGCTGGATTTCCAGCTGATCTGCTACGCCCGCCGCC11.0No Hit
AAGGAAACAGTCGCCGCAGATCGTTGACCTCGACTCGGGCCCCGAGGCAA11.0No Hit
CACCACACCCTACAGATTAGGCTTCCGTCCGCCGGTCGATTCGATCTTGC11.0No Hit
AGTGACATACATGAGAAGACACTCCGTACCCGTACTCCAGGCATATCGAT11.0No Hit
TTGTAGCGGGCATCGACACGGACCTTTTCGCAGGTACGACGAAAAGATCG11.0No Hit
ACCCAAGCTAATAGGAACCGTGTTACGAAGATCGGAAGAGCGTCGTGTAG11.0Illumina Single End PCR Primer 1 (100% over 22bp)
TCCGGCGGGTATCGATGACCAGACCTGGCTCGGAACGCTGCGCGACAGAT11.0No Hit
GGACGTCATCGAGGGCTGCGGCAAAGCAGTCCAGGAGCTCGCACGCTCTG11.0No Hit
GGCTTGACCTTTCCTCCCGCCGCCACGGCGGCGATCTGAAGACCGGCGCA11.0No Hit
TTGGGCAATAGCGCAGTGATGAGATCTTGTACGGTAGAGATGACGCCAGC11.0No Hit
TGTATGTAGATGGAATAAAAACGGAAAAAAAAATAATTGGACTGTGTGGG11.0No Hit
CTTGACCTTCGAAAAGTCAAGCAGTAGCCTAAGTCGAGTCTGTTGTGCTA11.0No Hit
TAGAGTTTGGCGGCTCTGTGTGTCAACTGGTTCTTTTCATGGCTGGAGTT11.0No Hit
CTTCCTGGGTGACTGTGAGTAATTTGATATGGGAAATATTTCTTCGCAGC11.0No Hit
GGCTCAGCTATATGACCCACCGATTGACGAATTCAGTGTCATTAGAACTG11.0No Hit
CGGCATCTCGAACACTTCATCCGGGACAACGGATGGCAGACCTACGGTGA11.0No Hit
CCGTGAACGCCGCGGCCAGGGCCCCTTGGGCGCGCAGATCGGAAGAGCGT11.0No Hit
GTTGTCGGCCGCGTATTGCACTCCCGCCTGCGACAACCCATATCCTTCTG11.0No Hit
CGTGCAGCCCACGACGGCATGAGGTAGACGAGCCAGAGCACGGCCGCGAC11.0No Hit
CTCTCGCCCGCCCCGCCGCCGACGCCCTGCTCGCGTCATTCAAGATCTGG11.0No Hit
CAGCTCGACCTGGCCTTCATCATCCTCGCGCTGGGCTGCTCGCTGCTCAT11.0No Hit
GCGAGGCCGCCGACTCCGGGTCCCTGGTGCGGTACAGATCGGAAGAGCGT11.0No Hit
CCCGGGGAGCTTGCTTGGTTGAGAAGATCGGAAGAGCGTCGTGTAGGGAA11.0Illumina Single End PCR Primer 1 (100% over 26bp)
CCCACCACCCCACGTTCCGGGTCTCAGTTTGCAGAGAGATCGGAAGAGCG11.0No Hit
CTGATGAAATTGTGTCTGTACATCCGTTGTCCTTAGATGCAAGATCGGAA11.0No Hit
GAAGAAAGTGGGCCAAGTGAGTATGCCAACAATAGACGCAGGAGAAGAGC11.0No Hit
AGCTTTTCCAAATATCCGCATACCAGTAGAGGAAGAGTCGGAAGCCATAA11.0No Hit
ATCTGCTCCTCCAACTCCCGTCGAGAGCGTGCCCCCGAATCTGCCAGCCC11.0No Hit
GACCAAACTCTTCGAGGAGCAGACGCCCATGCAGAGATCGGAAGAGCGTC11.0No Hit
CGCGGGCGCGCTGCTGCTGATCGCCGGCTTCCTGGCGCTTGGAGATCGGA11.0No Hit
TTGACCGGGACAGCTATAGATCTGGACGGCTTGTTGGTTGCTCGTGACTT11.0No Hit
CCACGAGAGACGCGTCCAATGTTAGTTGGAGCGGAACGGATATCGCGATC11.0No Hit
GTGCTATTCAGCCCGTAGGCTTCAGGTGGAAGCGCAAATCGACCTAGATC11.0No Hit
CTATGTTGTCCTGCTGTTTGTGTGACCGACGCGCAAGATTGTTGTAGAGA11.0No Hit
AGAGATTTGGCATTACTTGTGGTGAGCATTTACACACTCGGTAGCAACGG11.0No Hit
CGGCGCGGCCTTGATATCACACAGCGCGTTACGAGGAGGAAAACCAGGGG11.0No Hit
CCCTAGTCTGGTCGGTAGACCCTCCGGAGCTCCACCGCTTCATTTGAGAT11.0No Hit
AGTTAGGTCCAGCAGTCCATCTAAAATGTAAACCCTAGATGCACTACCTA11.0No Hit
ACGCCTACCACATCAACATCAGAGCTTCGAGCTTGAACACGACCGAGCAA11.0No Hit
ACACCAGTTTATTGCTCATGATGCAGCACTTCATCTCGCCGAGGAGTACG11.0No Hit
CACACGACATCAGCCAGGACCAAGATCGTTAATCTCACTCAGACCAACGC11.0No Hit
AATGTCTTACATATGTTGCTATGTGCTGGAGCAAAATTTGACCTCGCGCA11.0No Hit
CGTGATCCTTCTTCAAGTTGGGCCGAATCACAAAGCTCTCAGACTCAAAT11.0No Hit
GATCCGCCAGAAGGTCCGCGTCGACGGAGATCGGAAGAGCGTCGTGTAGG11.0Illumina Single End PCR Primer 1 (100% over 23bp)
ACAAAAGCTACGTCTGACGAAACGCTTGGTCGTCTCGCAAAGAGACCAAA11.0No Hit
GGCAGTCAGGGCGGTCAGCTGCTCGCCCACCGGCAGCACGTCGATGGGCA11.0No Hit
GTAACGTCATTGCCGTACTCGGTCAGCATGGGCGACGCTTCTTTAACGAT11.0No Hit
GTCTTCAGCTGGGACTCTCCCCGGACAAGAGAAGAGATCGGAAGAGCGTC11.0No Hit
TCGTGGGTATCTGTTTAGACTCGTGCAACTGCAGACCACCGCCGAGTTCG11.0No Hit
GCAAGAGACCGAAGGACTGATTGCCAAAGTAGGGGGATAGGTATTTGCAG11.0No Hit
ATCCATGGGCGGCTTCCAAAGGGTCAGGATTTAGATCGGAAGAGCGTCGT11.0No Hit
TCCTCATGAAGCTGGCATTCTCGGCACCGCCAGCTACGACCGCCGAGATC11.0No Hit
GGCTCGCGGACAAGGCATCGCGAACGCACTGCCTCGTGAGATCTTGTAGA11.0No Hit
CGAAGGCGAGCATGACGGCGACGTAGGCGCTCGTCGCGGGGGCAGATCGG11.0No Hit
GTTGTAGTGATCTATGTGAGCCACTCCTGTGTTGTGCGTGCTGCGATCCC11.0No Hit
CTTCTTGATTTGAGCAAGTCTCAATTAAACTTGGAGCGACAACACAGATC11.0No Hit
CTAGTCCAGTCATCATCGGCCTTCACCGTCTTCCTATTACAGTGACCCGT11.0No Hit
TCCTTCTTCTGGTTACGCTTCCGTTTCATCTGCTCCGTTGCTGCATCGAA11.0No Hit
ATTTCGGTAGCAGCGTGGATGTCTGCGCGTACGGGGTTCGGATCACGTCG11.0No Hit
AGCGAGTGTCGGTAACAGCCAAGCAGGAGGGAGGAAGATGAAGAAGTGGC11.0No Hit
GTTATGGCTGGGCTCCTGTAGCGCGATCACATCATAATCCTGCGTTTTTG11.0No Hit
TCGCCAGTATGAAGCGCCAGACGCCGGAGTTCTCCTGCGAGATCGGAAGA11.0No Hit

[FAIL]Adapter Content

Adapter graph