FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n01_HRBB6LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n01_HRBB6LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATG3848.10126582278481TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCGCGTATG22.5316455696202533TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATATCGTATG22.5316455696202533TruSeq Adapter, Index 1 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTAT22.5316455696202533TruSeq Adapter, Index 1 (97% over 36bp)
TGCATAGAGAGCGCCAAGTCGTTGCGAGATCGGAAGAGCACACGTCTGAA22.5316455696202533Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATC22.5316455696202533TruSeq Adapter, Index 1 (97% over 36bp)
CCCAATAATCGGGCTCCACCTTTTCTTCTGGTGCCGATGGTTCGGACTTC11.2658227848101267No Hit
GAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTCTTC11.2658227848101267TruSeq Adapter, Index 1 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGTCGTATG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGTGGTTTG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GTGACTGGAGATCGGAAGAGCGCACGTCTGAACTCCAGTCACAGCGCCAA11.2658227848101267TruSeq Adapter, Index 13 (97% over 35bp)
CTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
ACTCAATGGTTTTCAATAGGAGGAGTGTGTTGGGGGGGGGGGGGGTTTGC11.2658227848101267No Hit
AATGGGCGCTCCTCGTGTTCGGCATTCATGGGTGCACGGTCTTTGGCGCG11.2658227848101267No Hit
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATG11.2658227848101267TruSeq Adapter, Index 13 (97% over 35bp)
ACTTCTATGGCAAGGTGGAGGAGAAAGAGATCGGAAGAGCACACGTCTGA11.2658227848101267Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCGGGGAT11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GTGCACGATGGCATTGGGCGACAGTGTCTCGCCATCGATCGGCGTGGTGA11.2658227848101267No Hit
GATCGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGC11.2658227848101267TruSeq Adapter, Index 1 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTCTT11.2658227848101267TruSeq Adapter, Index 1 (96% over 29bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTC11.2658227848101267TruSeq Adapter, Index 1 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTAGTAT11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
CTCCAGGTACACCTGGGTACTAGTAAGGGTCCTAAGTTGAGTTCAGATCG11.2658227848101267No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCGCGGTTT11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCCGTG11.2658227848101267TruSeq Adapter, Index 1 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTTTG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
CGTCAGCGAAGACTTTGTAAATCTCGTCACGTGCAGTTTCATAATTAGAT11.2658227848101267No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCGGGTATG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATT11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
CCATTCACGGTTAATGCTGGCAGAATGGTTTTAGTAGAAGATCGGAAGAG11.2658227848101267No Hit
TGAATTGACGTATAAACAGTTAAAACAGATCGGAAGAGCACACGTCTGAA11.2658227848101267Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTATGCC11.2658227848101267TruSeq Adapter, Index 1 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATGGAGTATG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
CCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAA11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGAATG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)
GACGCTCTTCCGATCTTCGTAGTTACGAAGATCGGAAGAGCACACGTCTG11.2658227848101267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGCCAATCTCGTAAG11.2658227848101267TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)