FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n01_HRBB6EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n01_HRBB6EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6132
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGGTTG150.2446183953033268TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGGATG130.21200260926288322TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGGGTG110.17938682322243965TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGGTTT110.17938682322243965TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGGGTT90.14677103718199608TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGCGTTTG80.1304631441617743TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGGATT80.1304631441617743TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTTGAATCGGGTATG80.1304631441617743TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)