FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n01_HRBB5LB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n01_HRBB5LB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3785
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATG1985.231175693527081TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTAT320.8454425363276089TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCGCGTATG190.5019815059445178TruSeq Adapter, Index 18 (97% over 37bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATC90.23778071334214002TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTTTG70.18494055482166447TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTGGTATG50.13210039630118892TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCTCGTATGC50.13210039630118892TruSeq Adapter, Index 18 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCGCGTATT50.13210039630118892TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTCTATCGGGTATG40.10568031704095111TruSeq Adapter, Index 18 (97% over 37bp)
TAGGTTCCATGATTACTGGACGCGCTCGCTTAGCGCCGTCTTGGAAGGCC40.10568031704095111No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)