FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n01_HRBB5EB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n01_HRBB5EB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3672
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATG57915.76797385620915TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTAT972.64161220043573TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATGC140.3812636165577342TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATATCGTATG130.3540305010893246TruSeq Adapter, Index 3 (97% over 37bp)
CAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATC70.1906318082788671TruSeq Adapter, Index 3 (97% over 37bp)
CGCAACGACTTGGCGCTCTCTATGCAAGATCGGAAGAGCACACGTCTGAA70.1906318082788671Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATGCCGTC50.13616557734204793TruSeq Adapter, Index 3 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATGCCG50.13616557734204793TruSeq Adapter, Index 3 (97% over 34bp)
GTGACTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAA50.13616557734204793TruSeq Adapter, Index 3 (97% over 37bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATG50.13616557734204793TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATGC50.13616557734204793TruSeq Adapter, Index 10 (96% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATGTCGTATG50.13616557734204793TruSeq Adapter, Index 3 (97% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCTCGTATG40.10893246187363835TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGTAAATCGCGTATG40.10893246187363835TruSeq Adapter, Index 3 (97% over 37bp)
GTTCTGTAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG40.10893246187363835TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)