FastQCFastQC Report
Fri 7 Jan 2022
HF55KAFX3_n01_HRB0073.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF55KAFX3_n01_HRB0073.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACTTATGGACTGAATCATATGGCTTGCTAATTAGGTTTTATTTTGGTA22.0No Hit
TTCCAGCCTTCCCCCCGCTTCCGCAATTCGGCCGAATCGTTCGGCGACCA22.0No Hit
TTGATATTGACAGGTGCCCTCCGACCATCTTGCTTTGTTCTTATAGTCCT11.0No Hit
CAGTTCTAATGACACTGAATTCGTCAATCGGTGGGTCATATAGCTGAGCC11.0No Hit
ACCTCAATCGCCGGCTTAGCGAGATCCTTCATCCTCATCCACCATTGGGG11.0No Hit
AAACGATCCCCGACCTGCAGAAGATCGTTAAAGAAGCGTCGCCCATGCTG11.0No Hit
CCGTCGAACTCCATCGCTGAGACGGGTATTGGAAAGTTATAGATCGGAAG11.0No Hit
AGGGAGTGGTCACTGCCCGGCGGCGGGCGTAGCAGATCAGCTGGAAATCC11.0No Hit
CAAATGAAGCGGTGGAGCTCCGGAGGGTCTACCGACCAGACTAGGGAGAT11.0No Hit
AGCATCTAAGGACAACGGATGTACAGACACAATTTCATCAGAGATCGGAA11.0No Hit
GCCGTATACATCCTATCTGTCTCCTGATAATCAATGCGCCGCCTCTCGTG11.0No Hit
TGATCGGGGTCGACCACCTCTTGAAAGGCTTCGCAAGTGTAGAGTGCATT11.0No Hit
GTCGCGCAGCGTTCCGAGCCAGGTCTGGTCATCGATACCCGCCGGAAGAT11.0No Hit
ATTGGGTAGGAATTGTTTTTTTAATTAGGGTGTATACATGGCGTGTTGTA11.0No Hit
CCAAGCGCCAGGAAGCCGGCGATCAGCAGCAGCGCGCCCGCGAGATCGGA11.0No Hit
CACGCTCATGAGCAGCGAGCAGCCCAGCGCGAGGATGATGAAGGCCAGGT11.0No Hit
GCCCCCGCGACGAGCGCCTACGTCGCCGTCATGCTCGCCTTCGAGATCGG11.0No Hit
TCGTAACACGGTTCCTATTAGCTTGGGTAGATCGGAAGAGCACACGTCTG11.0Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGATATTTTCGATGCAGCAACGGAGCAGATGAAACGGAAGCGTAACCA11.0No Hit
GCCCGATCCTGGCTAAGCGCATCCTGGACGAAGTGGGCCGCCGCCTGATC11.0No Hit
GTTTAAAAGGGTTTGTATAGGAAAAATTTTTACAGCAAGATGGATGGAAT11.0No Hit
TCCTGCTCGTCGCGGCCGTGCTCTGGCTCGTCTACCTCATGCCGTCGTGG11.0No Hit
AATTCCAGCACCACCGGTATCTCCCTGGAAGTCACGAGCAACCAACAAGC11.0No Hit
GTTTTTGGACATATTGTACTGTATTGGGTGCGTTCGTGGCAAAAACTCAC11.0No Hit
ACACTCTTTCCCACACAGTCCAATTATTTTTTTTTCCGTTTTTATTCCAT11.0No Hit
TTTCGTCGTACCTGCGAAAAGGTCCGTGTCGATGCCCGCTACAAAGATCG11.0No Hit
ACAAGATCTCACGAGGCAGTGCGTTCGCGATGCCTTGTCCGCGAGCCAGA11.0No Hit
CGCAGGAGAACTCCGGCGTCTGGCGCTTCATACTGGCGAAGATCGGAAGA11.0No Hit
ATCAACATTCCGGACGTTCTTAATCAGTATACGTTCAATATGCCTGTGCT11.0No Hit
TTGCCTCGGGGCCCGAGTCGAGGTCAACGATCTGCGGCGACTGTTTCCTT11.0No Hit
GTACCGCACCAGGGACCCGGAGTCGGCGGCCTCGCAGATCGGAAGAGCAC11.0No Hit
GTATCTGTTGAAGCGCGATGAACTGCACATGTGGTAGCACAACAGACTCG11.0No Hit
AAGAACTCGGCGCGGAAGCGGATTACTACATCCCTGATCGCTACGCAGAA11.0No Hit
CCTGCGTACCGGGCTGGATTGGTAAGGTGGTTTTCTATTCCGCCGTCAGC11.0No Hit
CTGCATGGGCGTCTGCTCCTCGAAGAGTTTGGTCAGATCGGAAGAGCACA11.0No Hit
ACCTATTGCGGCAACCTGCCGCCGGAAAGCCCCGAGGTCGATCGCTGCTT11.0No Hit
TCATCTACAGCTCCTCCGCCGAACAGATCGGAAGAGCACACGTCTGAACT11.0Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCGCACGAACTCGGCGGTGGTCTGCAGTTGCACGAGTCTAAACAGAT11.0No Hit
CAAGTTGCAACTTCTTTTTCGGAAGCCAATCTCCAAAGAACTTCAGGGTT11.0No Hit
CCAATCATGCTCGTTCCAAGTACGAGAATAGATAGTCAGCATCACGTGAG11.0No Hit
CCAGCGCACTATGTCGTCTTCCACTAAGGGCGGCTGGCAAAACTTTGAGG11.0No Hit
TTCAGCTTCTTATCTGACCAAGTTACCGACACTGCAAATACCTATCCCCC11.0No Hit
CGATGCGATCCTCCATATCTAGAGACATCTTGCAAAGTGAGCCAGCATGG11.0No Hit
CTTCTCTTGTCCGGGGAGAGTCCCAGCTGAAGACAGATCGGAAGAGCACA11.0No Hit
CTTGGATGGTGTCCCTACATACCATTGCCAACCGTGCCGACAGCCAATTC11.0No Hit
AGCCTGCTCGGTATGATTAGAAGGGCAACCGTCACCCGCCAAACAACTGG11.0No Hit
CTCTGCAAACTGAGACCCGGAACGTGGGGTGGTGGGAGATCGGAAGAGCA11.0No Hit
GCAAATGATTTGCCGGCATCCACACTCTTCCACAGTCCATTCCCGCTTCG11.0No Hit
CAGCTCAATTTGAGTCTGAGAGCTTTGTGATTCGGCCCAACTTGAAGAAG11.0No Hit
TCTCATCAACAACCTTTCTAACCAAATATACAAAAGACTATATTCTATTA11.0No Hit
ATAACAAACCAGCTACGTGGGGTGCGGCCATTGAGGTTCCACTCAGAATA11.0No Hit
TTATCGTTGTCTGCCACTTCTTCATCTTCCTCCCTCCTGCTTGGCTGTTA11.0No Hit
GTTGTAGTATGGGATGGCTAAGAGCTCAACCAACAGCTAATTAGGTAGTG11.0No Hit
TCGGGCGTCCAGACCAGGGCCGTGGCATCCAGGGTCGACAGCAGGGCGAT11.0No Hit
CTACAACAATCTTGCGCGTCGGTCACACAAACAGCAGGACAACATAGAGA11.0No Hit
CCAGACTGGAGGGAAGATTGATGCCTTTACGTGTGCAGATCGGAAGAGCA11.0No Hit
TCGACTCTGATCTCAGGGATTTGTACGTCAGGTCCGTTGACAGAGCGTGC11.0No Hit
CGGCGGTCGTAGCTGGCGGTGCCGAGAATGCCAGCTTCATGAGGAAGATC11.0No Hit
TTTTTGGTTTTGTCATGGGCGGCAATTTTGCGTCTGCAAACCAATCCTCG11.0No Hit
CCAAGAGCAACTTGGGGTCGAGGGATCGATATGCCTGGAGTACGGGTACG11.0No Hit
CACGTCACCGTAGGTCTGCCATCCGTTGTCCCGGATGAAGTGTTCGAGAT11.0No Hit
CATTGGTACTTCATCTCGCTTCAGTTTACATACTTGGAGTCAGACCCCGT11.0No Hit
ACAGTTGATTGACCCGATGCGTGATGACGCGATTGATCTCTTTACTTATG11.0No Hit
GCCCTGCCCATCGACGTGCTGCCGGTGGGCGAGCAGCTGACCGCCCTGAC11.0No Hit
CCGTCGACGCGGACCTTCTGGCGGATCAGATCGGAAGAGCACACGTCTGA11.0Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTCTGCGCGTCATTCACACAGATCGGAAGAGCACACGTCTGAACTCCA11.0Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAAACCGCAGGATGCCGTACTCCTCGGCGAGATGAAGTGCTGCATCATG11.0No Hit
GCTATCGACAATTCCGATATAGCCTGCGTCAACGAGATTCCAGAGAATCT11.0No Hit
ATCCTCCGCGGTCGCGAGTGTTCGCTGTGACTTGCTCGGTCGTGTTCAAG11.0No Hit
TGATTATTTGCTATGATTTATGATATAGAATGGATAGGATAGATCGGAAG11.0No Hit
GCGCGCCCAAGGGGCCCTGGCCGCGGCGTTCACGGAGATCGGAAGAGCAC11.0No Hit
CGCTACCAAGCTGCGCTACATCCCTTTTCCAAATTGTTGTACAATGTCAT11.0No Hit
ATTGATGAGCGGTCAGAGAGGATTGCCTGTCCGGAGGCGGTAACGAAGTA11.0No Hit
GTGTTGTCGCTCCAAGTTTAATTGAGACTTGCTCAAATCAAGAAGAGATC11.0No Hit
TCCGCTTGCGGTCTGGGCTGGCAGATTCGGGGGCACGCTCTCGACGGGAG11.0No Hit
TTTGAAGCGCCAGTCCTCCCTTCTCGTGCCAAACGAGCTTGCGCCCGCTC11.0No Hit
CGTGTGTACGGAATCTGGGCCTCTTGTGTTGGAAACCTAATGATACAAGG11.0No Hit
AAATCCTGACCCTTTGGAAGCCGCCCATGGATAGATCGGAAGAGCACACG11.0No Hit
AGTAACTTGAGCAATGACTGAACTTTGTGAAAGGCTTGGAGGTGCAAACC11.0No Hit
AGGTCGATTTGCGCTTCCACCTGAAGCCTACGGGCTGAATAGCACAGATC11.0No Hit
AGCCATTCATCAGGGGATCGCAGCACGCACAACACAGGAGTGGCTCACAT11.0No Hit
TCTCAACCAAGCAAGCTCCCCGGGAGATCGGAAGAGCACACGTCTGAACT11.0Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGAGCCGCAGCTCTACTATTTCGGGCGCAACCTCCACGACCACATCG11.0No Hit
TCTTCTGCTCCGCGTTGGTCTGAGTGAGATGAACGATCTTGGTCCGGGCT11.0No Hit
ACTACGGCAAGATCGAATCGACCGGCGGACGGAAGCCTAATCTGTAGGGT11.0No Hit
CAGGAGGTGCTGATCCCGATCACGCTCGCGGTCATGCTGTCGTTTGTCCT11.0No Hit
TAGAACACTCACGGCACTTGTGGGGTCAAAAACTACAATCTAGCAGAGTG11.0No Hit
GTCGCTTGAGTCGCAGCAGAATGTGCACGACTCTTGAAAAAGTCGTTGTA11.0No Hit
CCTGCAAGCGAATGCTCATCTCGGGCTGTATGCTGATAACCAGGCGGTTT11.0No Hit
GGCGCGCGGGCCAGGCGGTCGGCCGGCGCCAGGCTGGCATAGGGATCCTC11.0No Hit
CTCCTGCGCCGGTCTTCAGATCGCCGCCGTGGCGGCGGGAGGAAAGGTCA11.0No Hit
CCCCGCCACGAGCCAGGCGATGCCACCGACGACGGGCAGCAGCACGATGA11.0No Hit
GCCGCCACTGAAGCTGAGGTCGAACTTTAATGCAGGGTCTCCACATCTTA11.0No Hit
TCGTCGCGCGAGTGGCCTGCTAGTCAGATGACGGCTCGAGGGAGGAAGCA11.0No Hit
TCAAACAGCAGTATGTCAATTGCCCAAGACAAGATCGGAAGAGCACACGT11.0No Hit
CGCATGCATCGACATTCATCAAGCTTTCTAGTTTGTCAAGTTCTTGCATA11.0No Hit
ACTAGAACAGGCAGGTGATTTGGTCTCTTTGCGAGACGACCAAGCGTTTC11.0No Hit
AGAGGGCGCCGCCATCACCGCCGCTGGCGTCATCTCTACCGTACAAGATC11.0No Hit

[FAIL]Adapter Content

Adapter graph