FastQCFastQC Report
Wed 13 Dec 2023
HF3T2DRX3_n01_NMD_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF3T2DRX3_n01_NMD_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28834698
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCCATATCTCGTAT1078990.3741984743519769TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCCATATCGCGTAT442660.15351643356902853TruSeq Adapter, Index 16 (97% over 39bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT437780.15182402812056503No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322660.11189990614779459No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT318030.1102942017981253No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)