FastQCFastQC Report
Wed 13 Dec 2023
HF3T2DRX3_n01_NMD_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF3T2DRX3_n01_NMD_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4599858
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT1605013.48925988584865TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCGCGTAT513321.1159474922921533TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTA206680.4493182180841234TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCGCGTA81350.17685328547098628TruSeq Adapter, Index 4 (97% over 38bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG68830.14963505395166546No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC60250.13098230423634818No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTA52180.11343828439921406TruSeq Adapter, Index 13 (97% over 35bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA48970.10645980810711983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)