FastQCFastQC Report
Wed 13 Dec 2023
HF3T2DRX3_n01_NMD_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF3T2DRX3_n01_NMD_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6261097
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG100200.16003585314202926No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC98860.15789565310999015No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTACCAATCTCGTAT73720.11774294504621155TruSeq Adapter, Index 15 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA68830.1099328120934718No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG68220.10895854192963311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)