FastQCFastQC Report
Wed 13 Dec 2023
HF3T2DRX3_n01_NMD_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF3T2DRX3_n01_NMD_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32267808
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG717060.2222214784468781No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC645760.20012515259790808No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA530720.1644735211018982No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTTT476290.14760531610947977TruSeq Adapter, Index 16 (97% over 38bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG391360.12128496611855381No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG390710.12108352696284792No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG375090.11624278909803851No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGGTT357510.11079463470217749TruSeq Adapter, Index 16 (97% over 38bp)
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC349050.10817282661406687No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTAT348550.10801787341737004TruSeq Adapter, Index 16 (97% over 38bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT325630.1009148188807867No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC322720.10001299127601106No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)