FastQCFastQC Report
Wed 13 Dec 2023
HF3T2DRX3_n01_NMD_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF3T2DRX3_n01_NMD_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34370424
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTAT1418360.4126687526461704TruSeq Adapter, Index 18 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG717100.2086386830723997No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC636540.18519992654149395No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA501800.14599761702095965No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCGCGTAT484500.14096421970238132TruSeq Adapter, Index 18 (97% over 37bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG360760.10496233622256158No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG358420.10428151831935503No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG347540.10111600601726647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)