FastQCFastQC Report
Wed 13 Dec 2023
HF3T2DRX3_n01_NMD_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF3T2DRX3_n01_NMD_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8963381
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTAT1866822.082718563452786TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGGAGATCTCGTAT700190.7811672849787373TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCGCGTAT677220.7555407942605585TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGGAGATCGCGTAT275310.3071497239713452TruSeq Adapter, Index 8 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTCGTA245310.2736802106258788TruSeq Adapter, Index 8 (97% over 36bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA211350.23579272151881084No Hit
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG137910.15385935284910904No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG126840.14150910242463197No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC114480.1277196629262998No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA99940.11149810545819708No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCGCGTA92300.10297453605955165TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTAGGAGATCTGGTAT92160.1028183449972728TruSeq Adapter, Index 8 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)