FastQCFastQC Report
Fri 5 Nov 2021
HF32FBGXK_n01_5QG3501.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF32FBGXK_n01_5QG3501.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4358385
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGTAT677861.555300874062296TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCGCGTAT109330.25084979872131535TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGGAT82900.19020807019113734TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCGCGGAT54130.12419738045170402TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGTTT46950.10772338836518573TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)