FastQCFastQC Report
Fri 5 Nov 2021
HF32FBGXK_n01_5QG3403-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF32FBGXK_n01_5QG3403-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96619
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTAT5800660.035810761858436TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGTAT66516.8837392231341665TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGAT28182.91661060453948TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTTT17321.7926080791562735TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGGAT16051.661163953259711TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTGGTAT9460.9791034889617984TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATATCGTAT8020.8300644800712076TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGTTT6710.6944803817054617TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTAA6390.661360601951997TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGGGTAT4620.4781668201906457TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGTT3660.3788074809302518TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCCTAT3330.3446527080594914TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTGGGAT3170.3280928181827591TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGGTT3110.32188285947898443TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTGGTTT2750.28462310725633677TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGGGGAT2650.27427317608337903TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTAGTAT2300.2380484169780271TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAACCAATCTCGTAT2270.23494343762613978TruSeq Adapter, Index 9 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGTAA1870.19354371293430897TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATAGCGTAT1700.1759488299402809TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAAAGCCAATCTCGTAT1600.1655988987673232TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGGGTTT1510.15628396071166129TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGGGGTT1340.13868907771763317TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCATCTCGTATG1260.13040913277926702TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGAA1230.1273041534273797TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTGT1200.1241991740754924TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCCTAT1190.12316418095819663TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAAGCTCGTAT1160.12005920160630933TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATATCGTAA1060.10970927043335163TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)