FastQCFastQC Report
Fri 5 Nov 2021
HF32FBGXK_n01_5QG3394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF32FBGXK_n01_5QG3394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362750
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGGGGTT230460.9753888477409798TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGGGGGT219910.9307374880964977TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGGGGAT196700.8325044968786373TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGGGTAT109460.463273727647868TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGGGTTT90780.38421331076076604TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGGAT65630.2777695481959581TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGTAT60200.2547878531372341TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGGGTGT41700.17648926039572532TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTAT36370.15393080097344197TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTGGTAT35410.14986773886361232TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGGTT34760.1471167072267485TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTGGGAT28440.12036821500370332TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGTTT26370.11160723732938313TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)