FastQCFastQC Report
Fri 5 Nov 2021
HF32FBGXK_n01_5QG3341-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF32FBGXK_n01_5QG3341-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83941
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTAT7346587.51980557772721TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATATCGTAT10711.275896165163627TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAAGCTCGTAT3820.45508154537115353TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCACTCTCGTAT3230.3847940815572843TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCATCTCGTATG1090.12985311111375847TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCGCGTAT890.10602685219380278TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTA860.10245291335580944TruSeq Adapter, Index 4 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)