FastQCFastQC Report
Fri 5 Nov 2021
HF32FBGXK_n01_5QG327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF32FBGXK_n01_5QG327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500640
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT911486.0739417848384685TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGTAT108200.7210256957031667TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGGAT39240.2614884316025163TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTGGTAT24680.16446316238404948TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCGCGGAT23860.1589988271670754TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTTT23150.15426751252798804TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)