FastQCFastQC Report
Fri 5 Nov 2021
HF32FBGXK_n01_5QG3195-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF32FBGXK_n01_5QG3195-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65908
Sequences flagged as poor quality0
Sequence length76
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATCTCGTAT5857488.87236754263519TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGCTCTCGTAT3980.6038720640893366TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATATCGTAT3050.46276628026946653TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGAGCTCGTAT2840.43090368392304423TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATCGCGTAT1450.22000364143958243TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTAAAGATCTCGTAT760.11531225344419493TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAAATCTCGTAT740.1122777204588214TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGAAGATCTCGTAT720.10924318747344784TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATCTAGTAT720.10924318747344784TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)