FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_Neg_Water_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_Neg_Water_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAAATAAAGTAGGTTACATCAGGGAGTACGGTGACTGGGCAGATGGTG24.545454545454546No Hit
ACCTTTGGGTAGGTTTCACCGCCGCGCCACATCCGAAAAATAAAACTTAT24.545454545454546No Hit
TTTTAATCCATATCGATAAGCTTTGATCGTACGTCTAGCAAGTTGATACG12.272727272727273No Hit
GTAAAAAAATAGTATGAAGTAAAATCCTCATGTAGTAAGGAATCAAATAG12.272727272727273No Hit
ACTGTGACATCATAAGTATGAGAAAAGAATCTTTGACGGCAGAAGATGTG12.272727272727273No Hit
ATGTATATCTGTATCTATATACATTTCTAAGTCCTGTCTTGACTTTCCTA12.272727272727273No Hit
CTCCTGCTTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCCCCAT12.272727272727273No Hit
GATTATATTCGAGAGAACTTAGATATTGAAGTCGTTGAGGAGGGTTGGAG12.272727272727273No Hit
GTTTTCCTAAATGGCAGGTCCCTGAAGACAACAGGATGACAGATTGATCA12.272727272727273No Hit
GTCCAAAATTGCACTTGAAACTCAATTTTCATAAAAACTTCTAGAAATTG12.272727272727273No Hit
CACCGACATATAAAATATTTATTTTTACCTAGACAGTGTAGTCTAATCAC12.272727272727273No Hit
CATTGATTCTTTCTTGATACAAATTCACATTGCTATGAGGTTCCTCAAAT12.272727272727273No Hit
GCTTAAAACCAAATAAACTTATTATCTTGTTTCCACGAGTCAAGAATCCA12.272727272727273No Hit
GTCTAGATGAATAGTTCCAGTCCCTACAACACTAAGAGATCTATCATCAC12.272727272727273No Hit
GAATAGAAAAACTCGTCAATTATCTAATGATTCAGTCTCAAAAACAATAA12.272727272727273No Hit
GCATGAGAAAGAAGTCTCTAGGAGCAAAGACCAGCCTGCCATCCACAGAC12.272727272727273No Hit
AGAGGGACCAGTTAAAAAGGAGAAAATAGCGGCCCAGAAACTAAACGAAA12.272727272727273No Hit
GGCAGATGCATGTTATTACTAAGGAATACAATCACATGCAAAACACTACT12.272727272727273No Hit
AGTTTCAACTCTCTTCTGTGTCTTCTGGGAAATGGTTGCTCTTCCTAACG12.272727272727273No Hit
ATTATATTCAATATGGAAAGAATAACAGAACTAAGAAATCTAATCTCGCA12.272727272727273No Hit
CTTTTATGCCCATTATTTTTTTTTATTCTCCTCCTCCTCCTGATTTTTAT12.272727272727273No Hit
AGAGACAGACAGACAGACTGAGCAAAGTGGAAGGAGCAGCAGGCAGTAGA12.272727272727273No Hit
GTGCCATCCTAGGCCACGCACGCTGCCGCAGGGTCGAGGCTGTGTCTGCT12.272727272727273No Hit
ACTTTACATAATTGCAATTTAAAATGTTTCCATTGTTCCAGGTGATATGG12.272727272727273No Hit
GTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCGTAGAA12.272727272727273No Hit
CCATTGCCACCACACCCCTCACCTCCATAGGCTTCACAGCCCCCCTTTAT12.272727272727273No Hit
GGGCCAGCTTTCTCAGCGGGGACGCGTTCCAGGGCCTCGGTCATGAGTAG12.272727272727273No Hit
CAGCGATATGTTAAAACAAAAAATCATTAACTAATTAATCAATTATTCAT12.272727272727273No Hit
ATACATCCCTGAGGGTAGGCCTCTAAAATGGCCGCTTCAGGGGGTGGCCA12.272727272727273No Hit
GTCTTGTGCATACAAGTAATGGGGAACATACGAAGGCTTTGGAATATTAT12.272727272727273No Hit
ATTATGGCCCATCCTCCCGCCGGTGACGACGTTCGCCACATTTCCAGCGA12.272727272727273No Hit
ATAGACGATAACCCTTTCAACAATAACTTCGATCTAACAGCAACAACTTC12.272727272727273No Hit
ACATAACACCAATCAACCAGATCACAATCAGAGGAGTGAGAATCACCAAA12.272727272727273No Hit
GCATACGCGCGGCTCGCCCAGGCGGTGGACGGCGTCGTGGTCTCCTCGCA12.272727272727273No Hit
GTGTCCTTTCCCGACTTTATTTTTTTATTTGCTTTGTCAAAGAACAGTTG12.272727272727273No Hit
TGTAACAGGTGATGTGTTGCAAGATGCTTTGATTCTTTCATTAGAAAATA12.272727272727273No Hit
CTGTTAGCTGTGAATAAGTCAGACGTGACCATGAACAGGATTCTCCTGAA12.272727272727273No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT12.272727272727273No Hit
ATAGAGCCCAAGGAAAAAACTCAACACCACAGTAACAAAAAAGCAGACAC12.272727272727273No Hit
CAAAAAAAGTCCAACATTTCCATGAAAAGCCATTAAGCTCTGGGGTGCTG12.272727272727273No Hit
CCTAAATCCTGAGAAGTCATATCCAACATTTGGACATAGTTTTTTCCTGA12.272727272727273No Hit
AAAACTGAAGGATGAGCTAATTCTCAGTCCCATTGCAGCCTTGCATATAT12.272727272727273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)