FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_3018_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_3018_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390687
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC46141.1809965522272305No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT15190.3888022893006422No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT15060.3854748174369764No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG13350.3417057644610647No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13160.3368425363526301No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC12830.32839587700640155No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC12790.32737203950988897No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC11230.2874423771458994No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA11090.28385894590810545No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT11060.283091067785721No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG9410.24085777105457823No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG9150.23420282732724662No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT8980.22985151796706826No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG8910.2280598023481713No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG8890.227547883599915No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG8790.22498828985863364No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG8780.2247323304845055No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT8400.21500587426763626No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC8320.21295819927461113No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA8160.2088628492885609No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC8040.20579133679902326No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT7910.20246386493535745No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA7670.19632083995628213No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA7430.19017781497720682No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT7410.18966589622895053No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT7150.18301095250161895No Hit
CAGTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATG7100.18173115563097825No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC6920.17712388689667177No Hit
ATCCAGCCATCTGTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATT6750.1727725775364934No Hit
GAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT6650.17021298379521202No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC6550.16765339005393062No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA6540.1673974306798025No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA6460.1653497556867774No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA6350.16253420257136786No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAG6300.1612544057007272No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT6280.1607424869524709No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC6200.1586948119594458No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC6190.15843885258531765No Hit
ACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTC6170.15792693383706138No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA6150.1574150150888051No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTC6130.15690309634054883No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT6080.15562329946990813No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC6060.15511138072165187No Hit
ACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCC6030.15434350259926746No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC6030.15434350259926746No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA6020.1540875432251393No Hit
GAAGAGGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGG5980.15306370572862676No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT5900.15101603073560166No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATT5770.14768855887193585No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG5730.1466647213754233No Hit
CTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACCAGCAC5640.14436108700827005No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA5560.14231341201524494No Hit
CTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACA5550.1420574526411168No Hit
GTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA5530.1415455338928605No Hit
CAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGAT5510.14103361514460425No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAG5490.14052169639634798No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG5440.13924189952570729No Hit
AGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGATCGCC5430.13898594015157914No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG5420.138729980777451No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC5400.13821806202919473No Hit
CTTTCAGTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAA5390.1379621026550666No Hit
GCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCC5330.13642634641029774No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC5290.13540250891378522No Hit
ATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCA5260.13463463079140078No Hit
GACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCATTCGCTTCTGG5230.13386675266901638No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC5150.13181907767599127No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC5150.13181907767599127No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT5150.13181907767599127No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC5150.13181907767599127No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA5130.13130715892773498No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA5100.13053928080535057No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT5090.13028332143122245No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT5080.1300273620570943No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG5020.12849160581232547No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT4990.12772372768994106No Hit
GGCAACACCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACA4960.12695584956755665No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG4950.1266998901934285No Hit
GTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTT4950.1266998901934285No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC4850.12414029645214711No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT4830.12362837770389085No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGC4820.12337241832976269No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT4800.12286049958150642No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC4780.12234858083325015No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAAC4730.12106878396260945No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA4710.12055686521435319No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT4670.11953302771784062No Hit
GTTCACAAGTGGCACACACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT4530.11594959648004668No Hit
CCTTTGGCATATCCGCAAGCGATTTGCAGACAATGGATTGGGTGATGCCC4500.11518171835766228No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT4490.11492575898353413No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA4490.11492575898353413No Hit
ATCCCAATGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCA4470.11441384023527786No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC4460.11415788086114972No Hit
ATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAGC4400.1126221246163809No Hit
GTCTGTGAGACCGATGCTGTGAATCAGCAATCTGTTCACAAGTGGCACAC4380.11211020586812462No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC4330.11083040899748392No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC4300.11006253087509951No Hit
GCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAG4280.10955061212684324No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT4230.10827081525620255No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACT4220.10801485588207439No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA4200.10750293713381813No Hit
AGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTA4190.10724697775968998No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT4170.10673505901143371No Hit
GTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACT4130.10571122151492116No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA4120.10545526214079301No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC4110.10519930276666487No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT4100.10494334339253675No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT4020.10289566839951163No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGA4010.10263970902538348No Hit
GATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGATCCAGCCATC3970.10161587152887094No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG3940.10084799340648652No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA3940.10084799340648652No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA3910.1000801152841021No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)