FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_3012_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_3012_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998743
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTGATGTCGTCATTGA59570.596449737319811No Hit
ATTATATACGTCCCTTGCGTAAAGATTGTGTGATAGCTACTCTTTATTCT54740.5480889478073939No Hit
GTGTAAGAAAAATGATTGTACTGTAAGCGCCTACATCTAATTTTCCTTCT50570.506336464936425No Hit
CTGTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAA41870.419226968299152No Hit
GTACTAACAGTTCCACTAAATCTCCCTTTTTTGACTCATGAAATTCTAGC39360.39409537789000776No Hit
ATATACGTCCCTTGCGTAAAGATTGTGTGATAGCTACTCTTTATTCTTTC35130.35174213986981634No Hit
GTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAAAT34300.34343169363890413No Hit
GTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAAGTGGTGGCGTCTT34240.34283093848968155No Hit
GTGGTGGAACGAGAAGGCTCATGGCTAGCTGGATTGGGCTTCATAGGTCT33420.334620618116973No Hit
AGTTTATAGACATCATGCCAGAAATTTTGTTGGGGCTAGCCATCAATACG30920.3095891535660325No Hit
ATACATGATGGGAAGCCATTGGGAAGATTTAAAGTAATGGATTACTCGGA30830.30868802084219865No Hit
CTTCTATGTGATTCTCTCAATGAGAGGATTGACTGTCAATAAATAAGCTC30040.3007780780441014No Hit
TCTCTGAAGCAAGCCTCATTAAGATTTATGGGCTTTGCAAATAATTCTGA29350.2938693938280419No Hit
CCATTATATACGTCCCTTGCGTAAAGATTGTGTGATAGCTACTCTTTATT27710.2774487530826249No Hit
GGCATAGATAGCATTATTGATCTTATCTCCTCTGTGCTACTGACTAGTAT27690.2772485013662173No Hit
CTTGTACTCCATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGATT27360.27394434804549317No Hit
GAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAAATAG26550.26583415353098844No Hit
CCATAGACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAAC26340.2637315105087094No Hit
GGCTAATAGGAGGGAGAAGTGATTCTAGGCAAAGGGAAGCAGCAGCAAAG26220.2625300002102643No Hit
ACCATAGACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAA24820.2485123800617376No Hit
AGACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAACAGGA24600.24630961118125486No Hit
ACATAAAGCTGAGCCAGATGTATTGTCTGGGTTAGAATTAATCTTTAGGC24230.24260495442771562No Hit
CAGTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAA24110.24140344412927048No Hit
GAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTC23630.2365974029354899No Hit
TACTTGTACTCCATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGA23520.23549601849524854No Hit
CCATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGATTTCCGTATG23160.2318914875999131No Hit
CATTAAGATTTATGGGCTTTGCAAATAATTCTGAAAGTTTTTATGGAGCC23050.23079010315967172No Hit
TATTTAACCTGATCTTGAAAGTGTTATTTGATGGGCTAATAGGAGGGAGA22960.22988897043583786No Hit
CCCCTACATGATAAAAATGCAAAAAAAGACCATACATCCTTTTTTTCAGT22630.22658481711511372No Hit
GGCTTATGGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAAGTGGT22340.22368116722720457No Hit
CTGTTAGTACAGATGCTTCATCCTTCTTATGTACGTGTTATCTGCAGATA22200.22227940521235193No Hit
CAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTGATG22110.22137827248851805No Hit
TATCTCCTCTGTGCTACTGACTAGTATCACTTTTTTTCTACTTGCTCCCA21660.21687260886934875No Hit
TTTACGTACTGTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAAAT21460.21487009170527352No Hit
ATTCAATAGTTGCTTGTTTAAAAAATTACCCTGCAAGGTATGAGAAACCA21330.2135684555486246No Hit
TTATACGCCTGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTCAGC21290.21316795211580958No Hit
GTGTTGGATGTGGGTGGGTTAGGCGGAAGACTTAGCTTCTAAGGTTCGTG21250.21276744868299452No Hit
TTTATGGGCTTTGCAAATAATTCTGAAAGTTTTTATGGAGCCACAAGGTT21080.21106530909353058No Hit
GCGTAAAGATTGTGTGATAGCTACTCTTTATTCTTTCCTTAACAAGTTTA21030.21056467980251176No Hit
GTAGTGGCTTATGGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAA20890.20916291778765908No Hit
CTCTTTATTCTTTCCTTAACAAGTTTATAGACATCATGCCAGAAATTTTG20500.20525800931771235No Hit
GTATGAGAAACCACCACCCTTGCTATGCCTCCCCTAGGGGAGGTTTGGTT20220.202454485288007No Hit
ATCCTGAAGAAACATTTATAAGCTTGTGGTTTAGTTGATATAATCATGGC19790.19814907338524526No Hit
GTTTATAGACATCATGCCAGAAATTTTGTTGGGGCTAGCCATCAATACGG19690.19714781480320762No Hit
GGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATCAAGTGGTGGCGTCT19460.19484492006452112No Hit
GGGTAGCATGACTAACAAAGCTATTTTGGGAGCAAGTAGAAAAAAAGTGA19350.1937435356242797No Hit
AGCTCGTACTTAGTGCACCCGCTTTTTTACGTACTGTGAGATAGAGTGGA18370.18393120152031103No Hit
AAACAGGAGAGACCAAGGCATGCCACCGAATGGCTCTAAAATTGGCTTCT18240.18262956536366212No Hit
CTAATGTATTACCAATATCTTTCTACATTCAAGTACAGAAGACATAAAGC18110.1813279292070132No Hit
ACATAGAAGTGACCAAGTCACCTGTTTATCATTTTCCTCAAACATGAGAT18070.18092742577419815No Hit
ATTCTATACATGATGGGAAGCCATTGGGAAGATTTAAAGTAATGGATTAC18060.18082729991599442No Hit
GCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAAAAAAG18030.18052692234138312No Hit
GTGCAGCAAAGTACGATGGCTTCTTTTTCCAAACAAAAAACTGGCAATTT17990.18012641890856806No Hit
GTAATGGATTACTCGGATGGTTAATAAAGCTAGAATTTCATGAGTCAAAA17620.17642176215502886No Hit
AGTCAGTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTT17390.17411886741634236No Hit
CCTCTGTGCTACTGACTAGTATCACTTTTTTTCTACTTGCTCCCAAAATA17310.17331786055071224No Hit
GATCAATAATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTG17280.17301748297610095No Hit
GGAATGGGTAGCATGACTAACAAAGCTATTTTGGGAGCAAGTAGAAAAAA17230.17251685368508213No Hit
GTGCTACTGACTAGTATCACTTTTTTTCTACTTGCTCCCAAAATAGCTTT17190.1721163502522671No Hit
ACCAATATCTTTCTACATTCAAGTACAGAAGACATAAAGCTGAGCCAGAT17060.1708147140956182No Hit
GATTTAAAGTAATGGATTACTCGGATGGTTAATAAAGCTAGAATTTCATG17030.17051433652100692No Hit
ATATAATCATGGCCGATTCAATAGTTGCTTGTTTAAAAAATTACCCTGCA16910.16931282622256177No Hit
TTGCTGCACGTGCCTAAAAGCAGCCATACGGAAATCGTGCTGCAAATGTT16640.1666094280510602No Hit
GTCTAAATGACGGCTCTCCTACTAAGTCCTGCTGCAGAGAATACATGCTG16620.16640917633465266No Hit
TTCAAGTACAGAAGACATAAAGCTGAGCCAGATGTATTGTCTGGGTTAGA16460.16480716260339245No Hit
GTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAACCCACCATAGA16400.1642064074541699No Hit
GACCAAGGCATGCCACCGAATGGCTCTAAAATTGGCTTCTTTATACGCCT15890.15909998868577802No Hit
ATACTAGTCAGTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCC15620.15639659051427643No Hit
GTAAACTATTGGCCAAGCTCGTACTTAGTGCACCCGCTTTTTTACGTACT15410.15429394749199743No Hit
TCTCTATACTGGTATTGTTAGTGTCTCACTTGAAATGAACCTGTTGATAA15390.15409369577558993No Hit
GCATTATTGATCTTATCTCCTCTGTGCTACTGACTAGTATCACTTTTTTT15120.15139029760408834No Hit
CTATACATGATGGGAAGCCATTGGGAAGATTTAAAGTAATGGATTACTCG15020.1503890390220507No Hit
GTATATAATGGAGCAACTGAAAAAAAGGATGTATGGTCTTTTTTTGCATT14860.14878702529079052No Hit
AGCAGGCGCTGTGTAAGAAAAATGATTGTACTGTAAGCGCCTACATCTAA14700.14718501155953034No Hit
GTGTGATAGCTACTCTTTATTCTTTCCTTAACAAGTTTATAGACATCATG14700.14718501155953034No Hit
GATGTATTGTCTGGGTTAGAATTAATCTTTAGGCAGGCAGATGGCTTTTT14570.14588337540288143No Hit
TTCCACCACCGTATTGATGGCTAGCCCCAACAAAATTTCTGGCATGATGT14450.14468186510443629No Hit
CTCGTACTTAGTGCACCCGCTTTTTTACGTACTGTGAGATAGAGTGGACC14370.14388085823880617No Hit
GGCTAAGGGATTTGAATTTTATTCTATACATGATGGGAAGCCATTGGGAA14210.142278844507546No Hit
ATCAAGTGGTGGCGTCTTGCTCAACATTTGCAGCACGATTTCCGTATGGC14170.14187834107473093No Hit
CTATTAAGATCCTGTAAATGACATACAGCCAGGTGTATTGTTATTTCGTC14050.1406768307762858No Hit
TTATTGATCTTATCTCCTCTGTGCTACTGACTAGTATCACTTTTTTTCTA14000.140176201485267No Hit
GTGTATAGTTTAGATGTCAAACTTCCTCTCTATACTGGTATTGTTAGTGT13950.13967557219424817No Hit
GGATTACTCGGATGGTTAATAAAGCTAGAATTTCATGAGTCAAAAAAGGG13920.13937519461963688No Hit
CAATACGGTGGTGGAACGAGAAGGCTCATGGCTAGCTGGATTGGGCTTCA13880.13897469118682185No Hit
CTATTGGCCAAGCTCGTACTTAGTGCACCCGCTTTTTTACGTACTGTGAG13850.13867431361221055No Hit
GCTAATAGGAGGGAGAAGTGATTCTAGGCAAAGGGAAGCAGCAGCAAAGG13760.1377731808883767No Hit
TCTTTATACGCCTGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTC13710.13727255159735788No Hit
GGTTAATAAAGCTAGAATTTCATGAGTCAAAAAAGGGAGATTTAGTGGAA13410.134268775851245No Hit
CTGTAGTGGCTTATGGTCTAGGGTCTTTGTTAGAATACTTGTACTCCATC13200.132166132828966No Hit
ATGATAAACAGGTGACTTGGTCACTTCTATGTGATTCTCTCAATGAGAGG13180.1319658811125585No Hit
GCTTTTAGGCACGTGCAGCAAAGTACGATGGCTTCTTTTTCCAAACAAAA13100.13116487424692838No Hit
ATCTCACAGTACGTAAAAAAGCGGGTGCACTAAGTACGAGCTTGGCCAAT13020.1303638673812983No Hit
GTAGCACAGAGGAGATAAGATCAATAATGCTATCTATGCCTTCTTCAAAA12970.1298632380902795No Hit
TGCTATGCCTCCCCTAGGGGAGGTTTGGTTTTTTTGGCACTTGTTCATCT12930.12946273465746444No Hit
GTATCAATGCATAGTGAGCTATCCTGAAGAAACATTTATAAGCTTGTGGT12900.12916235708285315No Hit
TTATTAGGCTAAGGGATTTGAATTTTATTCTATACATGATGGGAAGCCAT12890.12906223122464938No Hit
CTTTATACGCCTGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTCA12820.12836135021722306No Hit
CTATACTGGTATTGTTAGTGTCTCACTTGAAATGAACCTGTTGATAAGCT12810.1282612243590193No Hit
CCCTAGGGGAGGTTTGGTTTTTTTGGCACTTGTTCATCTAGTATTTTTGC12740.12756034335159294No Hit
TGTTAGTACAGATGCTTCATCCTTCTTATGTACGTGTTATCTGCAGATAA12400.12415606417266505No Hit
CTTTAAAACATCCAATCTCATGTTTGAGGAAAATGATAAACAGGTGACTT12350.12365543488164622No Hit
GATCTTGAAAGTGTTATTTGATGGGCTAATAGGAGGGAGAAGTGATTCTA12210.12225367286679356No Hit
GTACTGTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAACCCACC12180.12195329529218228No Hit
GCCTCAAGCTGCTCAGCTACTAAACTCTTGATGGTAAGCATGCCCAGTAA11910.1192498971206807No Hit
ACCCTAGACCATAAGCCACTACAGTCACTACCCCCAGCAAAAGCATCTGT11780.1179482609640318No Hit
GACTAGTATCACTTTTTTTCTACTTGCTCCCAAAATAGCTTTGTTAGTCA11680.11694700238199417No Hit
CCCTAGACCATAAGCCACTACAGTCACTACCCCCAGCAAAAGCATCTGTA11660.11674675066558665No Hit
AGTCAAAGGAATGGGTAGCATGACTAACAAAGCTATTTTGGGAGCAAGTA11640.11654649894917912No Hit
AACTGATTGATGTCGTCATTGAGAATGGAAAGCAAATTGCCAGTTTTTTG11610.11624612137456783No Hit
CCCTTGCTATGCCTCCCCTAGGGGAGGTTTGGTTTTTTTGGCACTTGTTC11530.11544511450893773No Hit
GAATAAAGAGTAGCTATCACACAATCTTTACGCAAGGGACGTATATAATG11510.11524486279253021No Hit
TGATTGTACTGTAAGCGCCTACATCTAATTTTCCTTCTACAACAAGAAAC11480.11494448521791893No Hit
GACTATGGTTAGTAAAAACCCCCAGAGAACGGCAAAGCGCAAAACAGGAG11420.11434373006869636No Hit
ATGCTATCTATGCCTTCTTCAAAAAAGCGCTCTAACTGATTGATGTCGTC11420.11434373006869636No Hit
TTTTTACGTACTGTGAGATAGAGTGGACCTAGTTTTCCTTGAACAAAAAA11340.11354272320306626No Hit
ATTCTAGGCAAAGGGAAGCAGCAGCAAAGGCTCGGAGGTAGGAAAAAGAA11300.11314221977025121No Hit
CCATAAGCCACTACAGTCACTACCCCCAGCAAAAGCATCTGTATTTTTAG11070.11083932503156467No Hit
GTGCATCCTCAGGATAAAAAGATAAAGATAAACCTGAGCAGTCTAAATGA11020.11033869574054588No Hit
GTAAAAACCCCCAGAGAACGGCAAAGCGCAAAACAGGAGAGACCAAGGCA10980.10993819230773082No Hit
GATTGATGTCGTCATTGAGAATGGAAAGCAAATTGCCAGTTTTTTGTTTG10960.1097379405913233No Hit
TTTATAAGCTTGTGGTTTAGTTGATATAATCATGGCCGATTCAATAGTTG10940.10953768887491577No Hit
GGGTAGTGACTGTAGTGGCTTATGGTCTAGGGTCTTTGTTAGAATACTTG10930.10943756301671202No Hit
GAGTACAAGTATTCTAACAAAGACCCTAGACCATAAGCCACTACAGTCAC10920.10933743715850824No Hit
CTATAAACTTGTTAAGGAAAGAATAAAGAGTAGCTATCACACAATCTTTA10910.10923731130030448No Hit
ATATAATGGAGCAACTGAAAAAAAGGATGTATGGTCTTTTTTTGCATTTT10860.10873668200928567No Hit
CATAAGAAGGATGAAGCATCTGTACTAACAGTTCCACTAAATCTCCCTTT10850.1086365561510819No Hit
CTCTAACTGATTGATGTCGTCATTGAGAATGGAAAGCAAATTGCCAGTTT10850.1086365561510819No Hit
TCTTTATTCTTTCCTTAACAAGTTTATAGACATCATGCCAGAAATTTTGT10830.10843630443467438No Hit
CCCCCAGAGAACGGCAAAGCGCAAAACAGGAGAGACCAAGGCATGCCACC10800.1081359268600631No Hit
ATACGGTGGTGGAACGAGAAGGCTCATGGCTAGCTGGATTGGGCTTCATA10790.10803580100185933No Hit
ATAGTGAGCTATCCTGAAGAAACATTTATAAGCTTGTGGTTTAGTTGATA10750.1076352975690443No Hit
CTTTTAGGCACGTGCAGCAAAGTACGATGGCTTCTTTTTCCAAACAAAAA10690.10703454241982173No Hit
GCAAAGTACGATGGCTTCTTTTTCCAAACAAAAAACTGGCAATTTGCTTT10650.10663403898700666No Hit
TGGCTGGCCCGTTCGATCTTTGTGGCCTCAAGCTGCTCAGCTACTAAACT10640.1065339131288029No Hit
GTACGATGGCTTCTTTTTCCAAACAAAAAACTGGCAATTTGCTTTCCATT10640.1065339131288029No Hit
GTGTTATTTGATGGGCTAATAGGAGGGAGAAGTGATTCTAGGCAAAGGGA10570.10583303212137658No Hit
GTACAGAAGACATAAAGCTGAGCCAGATGTATTGTCTGGGTTAGAATTAA10570.10583303212137658No Hit
GTCAAATATTGAAGCAAAGTTTTAGATTAATGTGTTAGATAGCATTGCCA10560.1057329062631728No Hit
CTTCAAAAAAGCGCTCTAACTGATTGATGTCGTCATTGAGAATGGAAAGC10530.10543252868856151No Hit
GAGTGGACCTAGTTTTCCTTGAACAAAAAAATTTCCGTAAATAGTCAAAG10380.10393064081550508No Hit
GTATAGTTTAGATGTCAAACTTCCTCTCTATACTGGTATTGTTAGTGTCT10360.10373038909909757No Hit
GAATTAATCTTTAGGCAGGCAGATGGCTTTTTATTTTTAAACAATTTATG10320.10332988566628251No Hit
GGTCTAAAAATACAGATGCTTTTGCTGGGGGTAGTGACTGTAGTGGCTTA10310.10322975980807875No Hit
GTATTGTCTGGGTTAGAATTAATCTTTAGGCAGGCAGATGGCTTTTTATT10240.10252887880065242No Hit
GTTAGAATACTTGTACTCCATCAAGTGGTGGCGTCTTGCTCAACATTTGC10140.10152762021861479No Hit
GTTTATATTTAACCTGATCTTGAAAGTGTTATTTGATGGGCTAATAGGAG10030.10042623577837342No Hit
GTAGGTAGGGTGGTGGTCATCAACAATTTATTAGGCTAAGGGATTTGAAT10020.10032610992016966No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)