FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_3000_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_3000_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2341663
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA65550.27992926394617845No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG60310.2575520047077654No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG34710.14822799010788487No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC32700.13964434677406612No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG32460.13861943413719224No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC28270.12072616768510243No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT27770.11859093302494851No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA26760.1142777590114376No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT26290.11227063843089291No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG25560.1091531958270682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)