FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_2856_d10_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_2856_d10_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2572503
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC89320.34721047944356137No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG79800.3102037198790439No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA64890.2522446037963804No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT63030.24501429152852303No Hit
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT58080.22577233146083794No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA56220.21854201919298052No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG52150.20272085202621726No Hit
GAGCAAAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATC49360.19187538362443116No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT48370.18802699161089412No Hit
GTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGC46070.17908628289257583No Hit
GAGCAAAAGCAGGACGGATAAGCAAAAGTAGGCTGGATAAGACGCGCCAG41260.16038853987731017No Hit
GATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA38280.14880449119009775No Hit
CATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTA37900.14732733061924513No Hit
CGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTG37380.14530595299597318No Hit
AATCTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCT35780.13908632953975175No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT34690.1348492110602009No Hit
GTTTAGAACGTCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGA33770.13127292757287357No Hit
GGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCATTAACCC32000.12439246912442861No Hit
CATCACTGGTGTTCGGGTTGTCATGCCAATGGCACTGCCCGGTAGCTAAA30790.11968887888566117No Hit
TCATTAACCCGCTTGTCGCCGGTTAATCTAATCTGTATCAGGCTGAAAAT30510.11860044478082242No Hit
AATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGC29620.11514077923329925No Hit
ATTTAAAGTGGTACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGT28850.11214758544499269No Hit
GGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCG28470.1106704248741401No Hit
GGAGCAAACGCGCCAGCGTCGCATCCGGTATTAATTGATATGCAAAAAAC27130.10546149022955464No Hit
GGATAAGACGCGCCAGCGTCGCATCCGGCATTTATTCCGCACCAACGCAA27000.10495614582373665No Hit
GTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGAT26720.1038677117188979No Hit
GGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTCAT26640.10355673054608683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)