FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_2856_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_2856_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339086
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG125120.9343686663888653No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA104130.7776199586882396No Hit
GCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACT93880.7010752110021312No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC82250.6142249265543811No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA68630.5125137593851329No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT61150.4566547630249289No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC58130.43410206663350975No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG56880.4247673413059355No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT53400.398779465993969No Hit
GCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT51320.3832464830488856No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG43510.3249231192022021No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC42960.32081584005806946No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC39980.2985618548771326No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA35810.26742121118434514No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT33710.2517388726340205No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA33170.24770627129250847No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA32960.246138037437476No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATCACTGCTGACAAAAGG32330.2414333358723786No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG31590.23590717847845472No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG31130.2324719995579074No Hit
GCCATACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATCTAAA27800.20760429128524976No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC26650.19901634398388157No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA26650.19901634398388157No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG25770.19244469735326933No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG24830.18542498390693354No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA24760.18490223928858937No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC23640.1765383253950829No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG23260.17370056889550037No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG22990.17168426822474434No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC22720.1696679675539883No Hit
GGACAAGCTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA22390.16720360006750873No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG22320.16668085544916458No Hit
GTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGCA21670.161826798278826No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT21340.1593624307923464No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC20840.15562854066131676No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA20780.1551804738455932No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG20630.1540603068062843No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT19990.1492809274385663No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT19950.14898221622808394No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA19070.1424105695974717No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT19040.1421865361896099No Hit
GGATCTAAAAGGCTAACAATTCTTGGAAAAGATGCCGGCACTTTAATTGA18010.1344947225196888No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG17790.13285181086203574No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA17790.13285181086203574No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC17760.13262777745417395No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCT17210.1285204983100413No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT16800.12545870840259699No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG16750.12508531938949405No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA16660.12441321916590868No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT16530.12344240773184097No Hit
TAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA16290.12165014046894673No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC15990.11940980639032893No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT15240.11380897119378441No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTC15200.11351025998330205No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC14690.10970169204965177No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT14350.1071626467605516No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA14220.10619183532648388No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC14090.10522102389241618No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA13450.10044164452469818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)