FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n02_2854_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n02_2854_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2785346
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC325101.1671799482003313No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG265250.9523053868352442No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCA236210.8480454492906806No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA146280.525177123416624No Hit
GAGCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCT145590.5226998728344702No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC140430.5041743467418411No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACG134740.483746004984659No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT131190.471000730250389No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA116370.4177936960076055No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT114500.411079987908145No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG104730.37600355575213995No Hit
GTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTC101170.3632223788355199No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGG95770.34383520036648946No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT85620.3073944852811823No Hit
GAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA84160.30215276665807406No Hit
CATTAGCCTTTTCCCCCTTAGCAAGGTTACTCAGTTCATTGATGCTTAAT82280.29540315637626346No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG79480.2853505453182477No Hit
AGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTCAATCC72780.26129608314371No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG72420.2600036045791079No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCG67640.24284236141578103No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC65300.23444125074586786No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG64780.2325743372636649No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC61930.22234221529389883No Hit
GGTATGGGGAGGATCTGAAAATTGAAACTAACAAATTTGCAGCAATATGC57330.20582721141287294No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG56420.2025601128190178No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG56240.2019138735367168No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG55100.19782102474881041No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA54780.1966721549136086No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTC54730.19649264400185829No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA54440.19545148071370666No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA53480.19200487120810125No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG51060.1833165430793876No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT50410.18098290122663396No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG50360.18080339031488368No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCGGAATCTAATG50230.18033666194433295No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG50220.1803007597619829No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT49410.17739268299162833No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATTCTATTGTATA48980.17584888915057592No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC44580.16005192891655112No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT44360.1592620809048499No Hit
TCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTCATCTGGGTCTTCAA43540.15631810195214527No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA42980.1543075797405421No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA42560.15279968808183975No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC42350.15204574225248854No Hit
TTTTTGGACAGTACGGATAACAAATAGCAGCACTGCTATAACTATTTTAA42290.1518303291583882No Hit
GCAAAAGCAGGTACTGATTCAGAATGGAAGATTTTGTGCGACAATGCTTC41640.14949668730563456No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTC41350.14845552401748294No Hit
ACAAATAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG40460.14526022978832792No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT40330.1447935014177772No Hit
CATTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA40040.14375233812962554No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC39770.14278297920617403No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTTCG39590.14213673992387302No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG39090.1403416308063702No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT39010.14005441334756974No Hit
TAGCAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA38730.13904915224176817No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA38710.13897734787706806No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA38580.13851061950651733No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG38540.1383670107771171No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA38420.13793618458891643No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT38170.13703863003016503No Hit
AGCTAATAGAGTCCTACAGACTTTTCCAATGGAACCTTCTTCCACTCCTT37740.1354948361891126No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA37020.13290987905990856No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG36910.13251495505405791No Hit
TGTTTGAAGAGTCGACAGACGCATTTGAAAAAAAGACGATCAAGAATCCA36890.1324431506893578No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC36700.13176100922470674No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA36500.13104296557770562No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA35760.12838620408380144No Hit
TTCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAA35470.1273450407956498No Hit
ATGTTCCTGAGAGAAATGAACAAGGACAAACTCTATGGAGTAAAATGAGT35450.1272732364309497No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT35120.12608846441339783No Hit
GTCTGGCTGTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAA35080.1259448556839976No Hit
GTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGCGTTTCCTGGG34840.12508320330759626No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT34780.12486779021349591No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG34470.12375482256064417No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT34400.12350350728419378No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC33830.12145708289024056No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC33610.12066723487853934No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC33460.12012870214328848No Hit
GCATGGAAAGAATAAAAGAACTTCGGAATCTAATGTCGCAGTCTCGCACT33010.11851310393753593No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA32920.11818998429638544No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCT32750.11757964719643448No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG32260.11582044026128172No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA32190.11556912498483132No Hit
CCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACCCTCTCAGGACGGC32120.11531780970838093No Hit
GTGGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAA31990.11485108133783019No Hit
ATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCTTTC31760.11402533114377891No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA31740.11395352677907879No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC31670.1137022115026284No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC31370.1126251460321267No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT30750.11039921072642322No Hit
CCTATAATGAGGAACCCTCTCAGGACGGCGGACTCCACTCCGGATGTGCT29340.10533700301506527No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCTAT29300.10519339428566506No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA29200.10483437246216448No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA29000.10411632881516335No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT28370.10185449132710982No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA28220.10131595859185898No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT28080.10081332803895818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)