FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_Neg_Water_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_Neg_Water_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGACCTGGGTGATGACGTACGCACCGAGGTGGTCGAGTTCACCCAG24.545454545454546No Hit
TAGTGACACTGTATTGTTACTTCAAATGGTATATTTTTTCAAACATATCA24.545454545454546No Hit
TTCAAGACCAAGGATGGATCATCAAGTCACTAAAATTATTGGATGTCAAT12.272727272727273No Hit
ACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCAAGTTAGGTTGCTTA12.272727272727273No Hit
ATTTCATTCACCATACATGGCACTGCATTAATGCTGCATTAAAAAACTTT12.272727272727273No Hit
CTGTTAAACTGTGAATAATTCAATTTAAGTATTTAGAAAGGAATATAGAA12.272727272727273No Hit
AGCAAGTTTTTATTAACAATTTTCAAAAGGGGAGGGAGTGTAAGAATAGG12.272727272727273No Hit
CATTTATGGTTATTATATCTTTCTGATGAATTGACCTTTTTCTCATTATG12.272727272727273No Hit
CCTTACCTCCTTCAATAGCCACTTATTTCCATTTTGTTGTTTTATGAAAA12.272727272727273No Hit
GCTTTATTGCTTATTTATAAAAGGCCAGTTTACACTGGAACAGGGCAGGG12.272727272727273No Hit
GATATTCAAGAAACAAAATAGGCCTAGGATACTCTAGACCACAGGAATCT12.272727272727273No Hit
GATGGAGGTGGCCTCTGCCTCGGCTTTCCTCCCAGAGGAGCCAAGTTTCC12.272727272727273No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT12.272727272727273No Hit
TGTGTACAGATACACCGTCAAACAGCTCACCAGTATCCTTGATGTGGTCG12.272727272727273No Hit
GGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTA12.272727272727273No Hit
ATAAGAATAAGAATCAGGGGCGCCTGGGTGGCTCAGTCACCTTTGGCTCA12.272727272727273No Hit
GTTTGTTGGCCTCGGGCCTCACGATGTAGCAGCAGAAGGGGCACAGAAGA12.272727272727273No Hit
GAAAAGAGGCATCTGTTATCTCAGCTGAAGAAGTGTAGATAGGAGGCTCC12.272727272727273No Hit
CTGACAGATTGATCACCACCTTGGTGGTGTTTAGTCACTCAATGTTTATT12.272727272727273No Hit
CAATAAGGCAAATCCTCGCCCGGTACTTAAGGGTCCAATCTGATAAAATG12.272727272727273No Hit
GTACTAGGAGTATCCAGTCTAAAAATTTATGAAACATGGTCCCTGGAGGA12.272727272727273No Hit
CCCTTACTCGTAGTAGTTATTAACTAAGTTATTCTATGGTACAGATAGTC12.272727272727273No Hit
AGCCAAGAAGCTGCCTCTCATCTTGGTGCTCAACAAGGTTGACCGTTCCG12.272727272727273No Hit
CCTCATTTGTAGGAACACAGATCTTACAAAGCCACAGTATTGGGCTCTAA12.272727272727273No Hit
CAAGTTCGATATCTAAGAGAAGAAGAGCAGAAAAAGTGCAAAGTTGTTGG12.272727272727273No Hit
CTCCGATTCTCTTAGCCTTGACAGCCCACCACTGCCTTCAAACAGGTGAT12.272727272727273No Hit
GTGAATGCTATGCTTTCACAGATTTTTTCAGCTTGGCCAGAGCCGCACAT12.272727272727273No Hit
TCATAGATGACACAAACAAATGGAACATCCTCATCGATCTGTAGAATCAA12.272727272727273No Hit
CATGGAGATAGCTAGTCAGGCCAGGCAGATGGTGCAGGCAATGAGAGCCA12.272727272727273No Hit
CTACTATGCTTTGGTGGACAGCCTGCCAAGGGAAGAATTATTATTAACTG12.272727272727273No Hit
TCAGAGAGCTGGGCCCATGCGACAAGACAGAGCTGATCTCCACTTAATTT12.272727272727273No Hit
GGTATATATAGGACATGCAGGACGAGGAGGACGCAGCAGGATATGAGGGT12.272727272727273No Hit
GTACCAAGAAGGCCTGAACAATAAGAATCCTTTTTTTTCTGCATTGGTTT12.272727272727273No Hit
AACCAAGTCAGGCGAGGCAGATCGTGCACGCAATGAGAGCCATAGGGACA12.272727272727273No Hit
CTAGAGCCCAAGGCAGATCCTTAACCCTGACTTAGCCACCCAGGTGTACC12.272727272727273No Hit
CACCCTGTCCTGCTTAAATTCCCTGTGCTGCAGGACAGACATATTTATTG12.272727272727273No Hit
TCCTAAGAAGTCATATCCTCAAAAGAATGGTCCTAAACCAAACAAGGGTC12.272727272727273No Hit
AGGGAAAACAACATCCATGATTACATCCAGTGCCTCCTTGGAACTGAGAT12.272727272727273No Hit
ATTTCGAATAATCAAAGTCTACTGATACAAATATCAGTGTGAATATCACG12.272727272727273No Hit
GGTAGAATGGCTATTAAATTAACATCTGTGCCAACTAGACACTGTTGAAG12.272727272727273No Hit
TCTCTCGAGTGAATAAACATTGAGTGGCTAAACACAACCAAGGTGTTGAT12.272727272727273No Hit
GAGTCCGGGCCCCACTGGCGGTGCAGCTCGGTGAGGGCCTCCAGGGCGAG12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)