FastQCFastQC Report
Fri 12 Nov 2021
HF2T7BGXK_n01_3018_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF2T7BGXK_n01_3018_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352240
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA167870.5007696346323652No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC136020.4057585375748753No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC134870.4023279956089063No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC134200.40032933202873305No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT130700.3898885521323056No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT126380.37700164666014363No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT106410.31742953965109894No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC104810.3126566116984464No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT100320.29926258263131517No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC95890.2860475383624084No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG92060.2746223420757464No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG84570.2522790730973916No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG79300.23655824165334224No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA77290.23056225091282245No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC73590.21952485502231342No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA73020.21782449943918095No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA72820.2172278834450994No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT71270.21260410949096725No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG69700.2079206739374269No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT69690.20789084313772282No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT67060.2000453428155502No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG64970.1938107056773978No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA63680.18996253251557169No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC63580.1896642245185309No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG63090.18820251533303106No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC63040.18805336133451064No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT62690.1870092833448679No Hit
GATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATATCAGGTGC59990.17895496742476674No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT59280.17683698064577716No Hit
GCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC58830.17549459465909362No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT56960.16991623511443096No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAA56400.16824571033100255No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT56050.16720163234135982No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA55160.16454669116769682No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG55090.16433787556976828No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG54280.16192158079373792No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC54060.1612653032002482No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT53940.16090733360379925No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC52410.15634322124907524No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA50400.15034723050855547No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC49230.1468570269431783No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG48920.14593227215235186No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC48690.14524616375915805No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA47040.1403240818079851No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT46150.13766914063432212No Hit
CATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATG45810.13665489344438345No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA45750.136475908646159No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC45400.13543183065651623No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG44460.13262773548433285No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC44150.13170298069350642No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTTGCAGGAAAGA44000.13125551869794524No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC43930.1310467031000167No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC43590.13003245591007803No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC43570.12997279431066988No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC43520.1298236403121495No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCT43130.12866023912369043No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT43050.1284215927260578No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT42100.12558766675417035No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT42070.1254981743550581No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG41520.12385748037133379No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG40540.12093406200033412No Hit
CTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGA40470.12072524640240555No Hit
GGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAG40370.12042693840536477No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA40330.12030761520654845No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA40210.1199496456100995No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT39360.11741402763525285No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG39220.11699639643939574No Hit
GTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACAACATGGATAGAGC39130.11672791924205904No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA39110.11666825764265087No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT38650.11529604085626327No Hit
GCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGCCTGTTCACTCGAT38590.1151170560580388No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT38590.1151170560580388No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT38120.11371500847194713No Hit
GCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACA37460.11174617569147793No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACT37380.1115075292938453No Hit
GATGAATACACAGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAA37370.11147769849414123No Hit
GCATGTACCATCTGCCTAGTCTGATTAGCAACCTCCATGGCCTCCGCTGC37160.11085125170035559No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC36950.11022480490656993No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC36640.1093000501157435No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT36530.10897191131899864No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT36480.10882275732047825No Hit
CAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATTTGCAGACAATGG36390.10855428012314153No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA36370.10849461852373338No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC36140.10780851013053958No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC35610.10622747774622342No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG35330.10539221535450924No Hit
GGATATGCAGCCGACCTGAAGAGCACACAGAATGCCATTGACGAGATTAC35240.10512373815717252No Hit
CAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAACCTCC35090.10467627616161133No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG34820.10387084456960122No Hit
GATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTT34800.10381118297019307No Hit
TATTTAAAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCA34130.10181251939001981No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA34090.10169319619120348No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATACATTT33670.10044030260363221No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC33610.10026131780540772No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT33600.10023148700570364No Hit
GACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAA33540.10005250220747917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)